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. 1970 Aug;20(2):245–250. doi: 10.1128/am.20.2.245-250.1970

Presumptive Identification of Group D Streptococci: the Bile-Esculin Test

Richard R Facklam 1, Max D Moody 1
PMCID: PMC376909  PMID: 4921062

Abstract

Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for each test are as follows: bile-esculin, 100:2; salt tolerance, 88:24; heat tolerance, 100:80; SF broth, 86:1; KF broth, 99:40; and methylene blue milk reduction, 90:17. These data indicate that the bile-esculin test provided a reliable means of identifying group D streptococci and differentiating them from non-group D streptococci. Methodology for reading and interpreting positive reactions and time of incubation of the bile-esculin medium was defined. Evidence of the need for standardization of salt and heat-tolerance tests was obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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