Abstract
A method has been devised for the determination of 9α-fluorohydrocortisone and 9α-fluoro-16α-hydroxyhydrocortisone in fermentation broths. The method involves extraction of the two steroids from the broth with ethyl acetate, separation through the formation of the water-soluble borate complex of the vicinally hydroxylated steroid, and estimation of each steroid spectrophotometrically.
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Selected References
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