Abstract
A rapid, semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy is presented. Results obtained by this method are compared with results obtained by the pseudoreplica method and a measureddrop method. A figure is presented which represents the limit of detectability for virus by negative staining.
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Selected References
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- BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
- FINK M. A., MALMGREN R. A., RAUSCHER F. J., ORR H. C., KARON M. APPLICATION OF IMMUNOFLUORESCENCE TO THE STUDY OF HUMAN LEUKEMIA. J Natl Cancer Inst. 1964 Sep;33:581–588. [PubMed] [Google Scholar]
- HALL C. E. Electron densitometry of stained virus particles. J Biophys Biochem Cytol. 1955 Jan;1(1):1–12. doi: 10.1083/jcb.1.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lampert F., Bahr G. F., Rabson A. S. Herpes simplex virus: dry mass. Science. 1969 Nov 28;166(3909):1163–1165. doi: 10.1126/science.166.3909.1163. [DOI] [PubMed] [Google Scholar]
- MOLONEY J. B. Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. I. Origin and introductory investigations. J Natl Cancer Inst. 1960 Apr;24:933–951. [PubMed] [Google Scholar]
- PULVERTAFT J. V. CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA). Lancet. 1964 Feb 1;1(7327):238–240. doi: 10.1016/s0140-6736(64)92345-1. [DOI] [PubMed] [Google Scholar]
- RHIM J. S., SMITH K. O., MELNICK J. L. Complete and coreless forms of reovirus (ECHO 10). Ratio of number of virus particles to infective units in the one-step growth cycle. Virology. 1961 Dec;15:428–435. doi: 10.1016/0042-6822(61)90110-6. [DOI] [PubMed] [Google Scholar]
- SMITH K. O., MELNICK J. L. Electron microscopic counting of virus particles by sedimentation on aluminized grids. J Immunol. 1962 Aug;89:279–284. [PubMed] [Google Scholar]
- WATSON D. H. Electron-microgrophic particle counts of phosphotungstate-sprayed virus. Biochim Biophys Acta. 1962 Sep 17;61:321–331. doi: 10.1016/0926-6550(62)90132-9. [DOI] [PubMed] [Google Scholar]
- WATSON D. H., RUSSELL W. C., WILDY P. Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology. 1963 Mar;19:250–260. doi: 10.1016/0042-6822(63)90062-x. [DOI] [PubMed] [Google Scholar]