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. 1970 Sep;20(3):346–350. doi: 10.21236/ad0868151

Concentration of Rift Valley Fever and Chikungunya Viruses by Precipitation

Frederick Klein 1, Bill G Mahlandt 1, Ralph R Cockey 1, Ralph E Lincoln 1
PMCID: PMC376937  PMID: 5494763

Abstract

Simple and efficient methods for concentrating Rift Valley fever (RVF) virus and chikungunya (CHIK) virus are described. Ammonium sulfate, potassium sulfate, or alcohol was used as a precipitating agent and the precipitate was resuspended to volumes suitable for further processing and purification. The methods permitted concentration of live RVF virus and CHIK virus about 100-fold with negligible losses of virus. RVF virus retained a high level of infectivity with potassium aluminum sulfate and alcohol, but CHIK virus retained a higher infectivity level with ammonium sulfate than with potassium aluminum sulfate. The data indicate that serum plays an important role in the concentration of both viruses, at least when the sulfate methods are used.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle J. J. Biological characteristics of plaque variants of Rift Valley fever virus. Am J Vet Res. 1967 Jul;28(125):1027–1031. [PubMed] [Google Scholar]
  2. FROMMHAGEN L. H., KNIGHT C. A. Column purification of influenza virus. Virology. 1959 Jun;8(2):198–208. doi: 10.1016/0042-6822(59)90004-2. [DOI] [PubMed] [Google Scholar]
  3. GROSSOWICZ N., MERCADO A., GOLDBLUM N. A simple method for concentration of live and formaldehyde-inactivated poliovirus. Proc Soc Exp Biol Med. 1960 Apr;103:872–874. doi: 10.3181/00379727-103-25703. [DOI] [PubMed] [Google Scholar]
  4. HOYER B. H., BOLTON E. T., RITTER D. B., RIBI E. Simple method for preparation of purified radioactive poliovirus particles: electron micrograph. Virology. 1959 Apr;7(4):462–464. doi: 10.1016/0042-6822(59)90074-1. [DOI] [PubMed] [Google Scholar]
  5. Hampil B., Melnick J. L., Wallis C., Brown R. W., Braye E. T., Adams R. R., Jr Preparation of antiserum to enteroviruses in large animals. J Immunol. 1965 Nov;95(5):895–908. [PubMed] [Google Scholar]
  6. MAYER M. M., RAPP H. J., ROIZMAN B., KLEIN S. W., COWAN K. M., LUKENS D. The purification of poliomyelitis virus as studied by complement fixation. J Immunol. 1957 Jun;78(6):435–455. [PubMed] [Google Scholar]
  7. MIDULLA M., WALLIS C., MELNICK J. L. ENTEROVIRUS IMMUNIZING ANTIGENS IN THE FORM OF CATION-STABILIZED AND CONCENTRATED VIRUS PREPARATIONS. J Immunol. 1965 Jul;95:9–12. [PubMed] [Google Scholar]
  8. MILLER H. K., SCHLESINGER R. W. Differentiation and purification of influenza viruses by adsorption on aluminum phosphate. J Immunol. 1955 Aug;75(2):155–160. [PubMed] [Google Scholar]
  9. MIMS C. A. Rift Valley Fever virus in mice. IV. Incomplete virus; its production and properties. Br J Exp Pathol. 1956 Apr;37(2):129–143. [PMC free article] [PubMed] [Google Scholar]
  10. POLSON A., LEVITT J. A SLOWLY SEDIMENTING INFECTIOUS COMPONENT OF RIFT VALLEY FEVER VIRUS. J Hyg (Lond) 1963 Dec;61:451–469. doi: 10.1017/s0022172400021070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TAVERNE J., MARSHALL J. H., FULTON F. The purification and concentration of viruses and virus soluble antigens on calcium phosphate. J Gen Microbiol. 1958 Dec;19(3):451–461. doi: 10.1099/00221287-19-3-451. [DOI] [PubMed] [Google Scholar]
  12. TAYLOR J., GRAHAM A. F. Purification of poliovirus labeled with radiophosphorus. Virology. 1958 Oct;6(2):488–498. doi: 10.1016/0042-6822(58)90096-5. [DOI] [PubMed] [Google Scholar]
  13. Walker J. S., Carter R. C., Klein F., Snowden S. E., Lincoln R. E. Evaluation of factors related to growth of Rift Valley fever virus in suspended cell cultures. Appl Microbiol. 1969 May;17(5):658–664. doi: 10.21236/ad0850080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weirether F. J., Walker J. S., Lincoln R. E. A precise method for replicating suspension cultures of mammalian cells. Appl Microbiol. 1968 Jun;16(6):841–844. doi: 10.1128/am.16.6.841-844.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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