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. 1970 Sep;20(3):374–383. doi: 10.1128/am.20.3.374-383.1970

Pectic Acid Lyases of Bacillus polymyxa1

C W Nagel 1, T M Wilson 1
PMCID: PMC376943  PMID: 5485720

Abstract

Four enzymes were separated from an extracellular preparation of Bacillus polymyxa by carboxymethylcellulose column chromatography. The pH optima were 8.3 to 8.5, 8.7 to 8.9, 9.2 to 9.4, and 9.5 to 9.6. All of the enzymes required calcium ion for maximum activity, whereas strontium ion was only partially effective in stimulating activity. Cobalt was the only other cation tested which was effective in two of the enzymes. The lyases seem to attack a calcium salt-bridged substrate. Km and Vm data of the four enzymes on various oligomers are presented as well as paper chromatographic evidence of preferred sites of attack. All of the enzymes are endo-enzymes which, based upon their characteristics, were classed into two types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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