Abstract
Mice were inoculated with St. Louis encephalitis (SLE), Flanders (FLAN), California (CE), or Tensaw (TEN) viruses. At fixed intervals after inoculation, brains from these mice were collected and assayed for infective virus and complement-fixing, hemagglutinating, and precipitating antigens. Detectability of these antigens was correlated with the appearance of signs of illness in the mice. Infective virus appeared 64, 48, 48, and 40 hr before signs of illness and 90, 86, 64, and 56 hr before death in mice inoculated with SLE, FLAN, CE, and TEN viruses, respectively. Diagnostic antigens were also detected well before signs of illness appeared. These findings were applied to the isolation of viruses from field-collected specimens. It was shown that by harvesting tissues at appropriate intervals these viruses could be detected and identified more rapidly than by conventional techniques with mice.
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Selected References
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- Ben-Ishai Z., Goldblum N., Becker Y. The intracellular site and sequence of Sindbis virus replication. J Gen Virol. 1968 May;2(3):365–375. doi: 10.1099/0022-1317-2-3-365. [DOI] [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- Coleman P. H., Lewis A. L., Schneider N. J., Work T. H. Isolations of St. Louis encephalitis virus from post-mortem tissues of human cases in the 1962 Florida epidemic. Am J Epidemiol. 1968 May;87(3):530–538. doi: 10.1093/oxfordjournals.aje.a120844. [DOI] [PubMed] [Google Scholar]
- Friedman R. M. Protein synthesis directed by an arbovirus. J Virol. 1968 Jan;2(1):26–32. doi: 10.1128/jvi.2.1.26-32.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hammon W. M., Sather G. History and recent reappearance of viruses in the California encephalitis group. Am J Trop Med Hyg. 1966 Mar;15(2):199–204. doi: 10.4269/ajtmh.1966.15.199. [DOI] [PubMed] [Google Scholar]
- Lesso J., Mayer V. Immunofluorescent and cytochemical study on tick-borne encephalitis virus-infected cells. Acta Virol. 1968 Mar;12(2):128–135. [PubMed] [Google Scholar]
- Luby J. P., Miller G., Gardner P., Pigford C. A., Henderson B. E., Eddins D. The epidemiology of St. Louis encephalitis in Houston, Texas, 1964. Am J Epidemiol. 1967 Nov;86(3):584–597. doi: 10.1093/oxfordjournals.aje.a120768. [DOI] [PubMed] [Google Scholar]
- Murphy F. A., Coleman P. H. California group arboviruses: immunodiffusion studies. J Immunol. 1967 Aug;99(2):276–284. [PubMed] [Google Scholar]
- Sudia W. D., Chamberlain R. W., Coleman P. H. Arbovirus isolations from mosquitoes collected in South Alabama, 1959-1963, and serologic evidence of human infection. Am J Epidemiol. 1968 Jan;87(1):112–116. doi: 10.1093/oxfordjournals.aje.a120792. [DOI] [PubMed] [Google Scholar]
- THOMPSON W. H., KALFAYAN B., ANSLOW R. O. ISOLATION OF CALIFORNIA ENCEPHALITIS GROUP VIRUS FROM A FATAL HUMAN ILLNESS. Am J Epidemiol. 1965 Mar;81:245–253. doi: 10.1093/oxfordjournals.aje.a120512. [DOI] [PubMed] [Google Scholar]
- Tikasingh E. S., Spence L., Downs W. G. The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses. Am J Trop Med Hyg. 1966 Mar;15(2):219–226. doi: 10.4269/ajtmh.1966.15.219. [DOI] [PubMed] [Google Scholar]
- Westaway E. G., Reedman B. M. Proteins of the group B arbovirus Kunjin. J Virol. 1969 Nov;4(5):688–693. doi: 10.1128/jvi.4.5.688-693.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]