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. 1970 Oct;20(4):590–592. doi: 10.1128/am.20.4.590-592.1970

Immunofluorescent Identification of Type 12 Group A Streptococci

William F Vincent 1,2, Kathleen J Lisiewski 1,2, William W Ullmann 1,2
PMCID: PMC376995  PMID: 5498608

Abstract

The fluorescent antibody (FA) conjugate prepared by labeling streptococcal M type 12 antibody with fluorescein isothiocyanate was found to exhibit considerable nonspecific FA staining with other group A M-serotypes. The cross-reactions could be reduced sufficiently or eliminated by the addition of adsorbed homologous blocking serum (AHB) but not by preimmune serum. The AHB was prepared by adsorbing type 12 antiserum with untreated homologous cells. Comparative staining with unblocked and AHB-blocked FA conjugates enabled type 12 streptococci from clinical specimens to be rapidly and accurately identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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