Abstract
The bile tests for characterizing gram-negative anaerobic bacilli were reevaluated in prereduced anaerobically sterilized peptone-yeast-glucose broth, in thioglycollate broth, and on blood agar plates. Blood agar plates were unsatisfactory. The combination of 20% bile with 0.1% desoxycholate inhibited Fusobacterium, Bacteroides melaninogenicus, and B. oralis and sometimes Sphaerophorus necrophorus, but not B. fragilis or other Sphaerophorus species studied. Ten per cent bile with 0.05% desoxycholate was less satisfactory. There was no significant difference between fresh and commercial powdered bile. Desoxycholate (0.1% in thioglycollate broth) inhibited B. fragilis, Fusobacterium, B. melaninogenicus, B. oralis, and S. necrophorus, but not S. varius or S. mortiferus/S. ridiculosus. The bile and desoxycholate tests are simple to perform and helpful for characterization and classification of gram-negative anaerobic bacilli.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BEERENS H., CASTEL M. M. [Action of bile on the growth of certain gram-negative anaerobic bacteria]. Ann Inst Pasteur (Paris) 1960 Sep;99:454–456. [PubMed] [Google Scholar]
- BEERENS H., SCHAFFNER Y., GUILLAUME J., CASTEL M. M. LES BACILLES ANA'EROBIES NON SPORUL'ES 'A GRAM N'EGATIF FAVORIS'ES PAR LA BILE. LEUR APPARTENANCE AU GENRE EGGERTHELLA (NOV. GEN) Ann Inst Pasteur Lille. 1963;14:5–48. [PubMed] [Google Scholar]
- ENEROTH P. THIN-LAYER CHROMATOGRAPHY OF BILE ACIDS. J Lipid Res. 1963 Jan;4:11–16. [PubMed] [Google Scholar]
- Finegold S. M., Harada N. E., Miller L. G. Antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli. J Bacteriol. 1967 Nov;94(5):1443–1450. doi: 10.1128/jb.94.5.1443-1450.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOESCHE W. J., SOCRANSKY S. S., GIBBONS R. J. BACTEROIDES ORALIS, PROPOSED NEW SPECIES ISOLATED FROM THE ORAL CAVITY OF MAN. J Bacteriol. 1964 Nov;88:1329–1337. doi: 10.1128/jb.88.5.1329-1337.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]