Abstract
Human sera were treated with kaolin, acetone, and dextran sulfate to determine the best method for removing nonspecific hemagglutination inhibitors. Results indicated that on surveys for group A, group B, and some group C arbovirus HI antibodies, dextran sulfate treatment of sera could be used effectively. This method, however, has limited usefulness for detecting HI antibody for a number of arboviruses, particularly some members of the Bunyamwera supergroup since nonspecific inhibitors for these antigens were not completely removed. HI antibodies in sera drawn early after dengue and Venezuelan equine encephalitis infection were detected more readily after dextran sulfate treatment than after kaolin treatment. Kaolin, but not dextran sulfate, was shown to remove antibody from IgM fractions of sera.
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Selected References
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- Bidwell D. E., Mills G. L. Serum non-specific inhibitors of arbovirus haemagglutination. J Comp Pathol. 1968 Oct;78(4):469–476. doi: 10.1016/0021-9975(68)90046-7. [DOI] [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- CORNWELL D. G., KRUGER F. A. Molecular complexes in the isolation and characterization of plasma lipoproteins. J Lipid Res. 1961 Apr;2:110–134. [PubMed] [Google Scholar]
- Chappell W. A., Halonen P. E., Toole R. F., Calisher C. H., Chester L. Preparation of La Crosse virus hemagglutinating antigen in BHK-21 suspension cell cultures. Appl Microbiol. 1969 Sep;18(3):433–437. doi: 10.1128/am.18.3.433-437.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dold H. J., Northrop R. L. The nonspecific inhibitors of rubella-virus hemagglutination. Proc Soc Exp Biol Med. 1968 Jun;128(2):577–581. doi: 10.3181/00379727-128-33071. [DOI] [PubMed] [Google Scholar]
- Gaidamovich S. Y., Tsirkin Y. M. Investigations on the sensitivity to inhibitors of tick-borne encephalitis virus strains. Acta Virol. 1965 Nov;9(6):488–494. [PubMed] [Google Scholar]
- Liebhaber H. Measurement of rubella antibody by hemagglutination inhibition. II. Characteristics of an improved HAI test employing a new method for the removal of non-immunoglobulin HA inhibitors from serum. J Immunol. 1970 Apr;104(4):826–834. [PubMed] [Google Scholar]
- Mann J. J., Rossen R. D., Lehrich J. R., Kasel J. A. The effect of kaolin on immunoglobulins: an improved technique to remove the nonspecific serum inhibitor of reovirus hemagglutination. J Immunol. 1967 Jun;98(6):1136–1142. [PubMed] [Google Scholar]
- PORTERFIELD J. S., ROWE C. E. Hemagglutination with arthropod-borne viruses and its inhibition by certain phospholipids. Virology. 1960 Aug;11:765–770. doi: 10.1016/0042-6822(60)90119-7. [DOI] [PubMed] [Google Scholar]