Abstract
A plaque assay for the quantitation of pneumonia virus of mice is described. To obtain reproducible plaque formation, proteolytic enzymes had to be incorporated in the overlay medium. The plaque morphology observed in the presence of pancreatin and chymotrypsin was superior to that seen with trypsin. Although the plaque assay was found to be slightly less sensitive than the TCID50 determination described by Harter and Choppin, it enables cloning of the virus by plaque selection and permits further study of pneumonia virus of mice.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Came P. E., Pascale A., Shimonaski G. Effect of pancreatin on plaque formation by influenza viruses. Arch Gesamte Virusforsch. 1968;23(4):346–352. doi: 10.1007/BF01242130. [DOI] [PubMed] [Google Scholar]
- Harter D. H., Choppin P. W. Studies on pneumonia virus of mice (PVM) in cell culture. I. Replication in baby hamster kidney cells and properties of the virus. J Exp Med. 1967 Aug 1;126(2):251–266. doi: 10.1084/jem.126.2.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STYK B., RADA B. INTERACTION OF MYXOVIRUSES WITH DEXTRAN SULFATES. II. SPECTRUM OF INHIBITORY ACTIVITY. CHARACTERISTICS OF ANTIVIRAL EFFECTS OF DEXTRAN SULFATES IN TISSUE CULTURE. Acta Virol. 1964 Jul;8:312–326. [PubMed] [Google Scholar]
- TAKEMOTO K. K., FABISCH P. INFLUENCE OF ACID POLYSACCHARIDES ON PLAQUE FORMATION BY INFLUENZA A2 AND B VIRUSES. Proc Soc Exp Biol Med. 1963 Dec;114:811–814. doi: 10.3181/00379727-114-28806. [DOI] [PubMed] [Google Scholar]