Abstract
Bacteria from stirred settling aerosols were recovered and identified by a rapid fluorescent-antibody technique previously described by Danielsson. Calibrated air samples from Serratia marcescens aerosols were drawn through a Millipore aerosol filter holder fitted out with a nonfluorescent membrane filter (type HAB 047). With the aid of a high-power incident-light ultraviolet microscope, the individual bacterial cells, trapped on the filter, could be inspected after staining with appropriate conjugate on the filter surface.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brock T. D. Life at high temperatures. Evolutionary, ecological, and biochemical significance of organisms living in hot springs is discussed. Science. 1967 Nov;158(3804):1012–1019. doi: 10.1126/science.158.3804.1012. [DOI] [PubMed] [Google Scholar]
- CARTER C. H., LEISE J. M. Specific staining of various bacteria with a single fluorescent antiglobulin. J Bacteriol. 1958 Aug;76(2):152–154. doi: 10.1128/jb.76.2.152-154.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DANIELSSON D., LAURELL G. A MEMBRANE FILTER METHOD FOR THE DEMONSTRATION OF BACTERIA BY THE FLUORESCENT ANTIBODY TECHNIQUE. 2. THE APPLICATION OF THE METHOD FOR DETECTION OF SMALL NUMBERS OF BACTERIA IN WATER. Acta Pathol Microbiol Scand. 1965;63:604–608. doi: 10.1111/apm.1965.63.4.604. [DOI] [PubMed] [Google Scholar]
- Guthrie R. K., Reeder D. J. Membrane filter-fluorescent-antibody method for detection and enumeration of bacteria in water. Appl Microbiol. 1969 Mar;17(3):399–401. doi: 10.1128/am.17.3.399-401.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]