Abstract
Thiabendazole, 2-(4′-thiazolyl) benzimidazole (TBZ) inhibited the growth of Penicillium atrovenetum at 8 to 10 μg/ml. Oxygen consumption with exogenous glucose was inhibited at 20 μg/ml, but endogenous respiration required more than 100 μg/ml. TBZ inhibited completely the following systems of isolated heart or fungus mitochondria: reduced nicotinamide adenine dinucleotide oxidase, succinic oxidase, reduced nicotinamide adenine dinucleotide-cytochrome c reductase, and succinic-cytochrome c reductase at concentrations of 10, 167, 10, and 0.5 μg/ml, respectively. Cytochrome c oxidase was not inhibited. Antimycin A and sodium azide caused the usual inhibition patterns for both fungus and heart terminal electron transport systems. In the presence of antimycin, the fungicide inhibited completely succinate-dichloro-phenolindophenol reductase and succinate-2, 2-di-p-nitrophenyl-(3, 3-dimethoxy-4, 4-biphenylene-5, 5-diphenylditetrazolium)-reductase at 2 and 4 μg of TBZ per ml, respectively. Coenzyme Q reductase required 15 μg/ml. TBZ reduced the uptake by P. atrovenetum of glucose and amino acids and decreased the synthesis of various cell components. At 120 μg/ml, the incorporation of labeled carbon from amino acids-U-14C was decreased: lipid, 73%; nucleic acids, 80%; protein, 80%; and a residual fraction, 89%. TBZ did not inhibit peptide synthesis in a cell-free protein-synthesizing system from Rhizoctonia solani. Probably the primary site of inhibition is the terminal electron transport system and other effects are secondary.
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Selected References
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- CRANE F. L., GLENN J. L., GREEN D. E. Studies on the electron transfer system. IV. The electron transfer particle. Biochim Biophys Acta. 1956 Dec;22(3):475–487. doi: 10.1016/0006-3002(56)90058-0. [DOI] [PubMed] [Google Scholar]
- KIKUCHI G., BARRON E. S. G. Electron transport system in Fusarium lini. Arch Biochem Biophys. 1959 Sep;84:96–105. doi: 10.1016/0003-9861(59)90557-0. [DOI] [PubMed] [Google Scholar]
- RAMASARMA T., LESTER R. L. Studies on the electron transport system. XXIV. The reduction and oxidation of exogenous coenzyme Q. J Biol Chem. 1960 Nov;235:3309–3314. [PubMed] [Google Scholar]