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. 1970 Dec;20(6):977–983. doi: 10.1128/am.20.6.977-983.1970

One-Day Fluorescent-Antibody Procedure for Detecting Salmonellae in Frozen and Dried Foods 1

J M Goepfert 1, M E Mann 1, R Hicks 1
PMCID: PMC377093  PMID: 4923810

Abstract

The indirect fluorescent-antibody technique was used to examine 422 food samples for the presence of salmonellae. A cultural phase involving a 16-hr preenrichment in buffered nutrient broth-milk medium followed by a 4- to 5-hr subculture into fresh medium of the same composition was evaluated. This procedure yielded a sufficient population of salmonellae so that no false-negative results were obtained. Of the 31 false-positives obtained, 12 samples yielded positive cultural results upon extensive subculture of the original enrichment broths. Yeast cells and both vegetative and spore forms of bacilli were observed to fluoresce when stained with anti-Salmonella serum. Efforts to ascertain the cause of these cross-reactions and several alternate explanations are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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