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. 1971 Jan;21(1):84–89. doi: 10.1128/am.21.1.84-89.1971

Indirect Hemagglutination Test for Detection of Antibodies to Cytomegalovirus

Michael T Bernstein 1, John A Stewart 1
PMCID: PMC377122  PMID: 4322281

Abstract

An indirect hemagglutination test has been adapted for use with cytomegalovirus. The test is highly sensitive and reproducible. Both immunoglobulin M and immunoglobulin G antibodies can be detected by this method. The hemagglutination reaction can be inhibited by small amounts of homologous antigen. This principle permits early identification of virus isolated from diagnostic specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benyesh-Melnick M., Vonka V., Probstmeyer F., Wimberly I. Human cytomegalovirus: properties of the complement-fixing antigen. J Immunol. 1966 Feb;96(2):261–267. [PubMed] [Google Scholar]
  2. Fuccillo D. A., Moder F., Traub R. G., Hensen S., Sever J. L. Micro indirect hemagglutination test for Cytomegalovirus. Appl Microbiol. 1971 Jan;21(1):104–107. doi: 10.1128/am.21.1.104-107.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hanshaw J. B., Steinfeld H. J., White C. J. Fluorescent-antibody test for cytomegalovirus macroglobulin. N Engl J Med. 1968 Sep 12;279(11):566–570. doi: 10.1056/NEJM196809122791102. [DOI] [PubMed] [Google Scholar]
  4. Ishizaka T., Ishizaka K., Salmon S., Fudenberg H. Biologic activities of aggregated gamma-globulin. 8. Aggregated immunoglobulins of different classes. J Immunol. 1967 Jul;99(1):82–91. [PubMed] [Google Scholar]
  5. KISSLING R. E., REESE D. R. ANTI-RABIES VACCINE OF TISSUE CULTURE ORIGIN. J Immunol. 1963 Sep;91:362–368. [PubMed] [Google Scholar]
  6. Lang D. J., Noren B. Cytomegaloviremia following congenital infection. J Pediatr. 1968 Dec;73(6):812–819. doi: 10.1016/s0022-3476(68)80233-1. [DOI] [PubMed] [Google Scholar]
  7. Leonard L. L., Schmidt N. J., Lennette E. H. Demonstration of viral antibody activity in two immunoglobulin G subclasses in patients with varicella-zoster virus infection. J Immunol. 1970 Jan;104(1):23–27. [PubMed] [Google Scholar]
  8. PLUMMER G., BENYESH-MELNICK M. A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS. Proc Soc Exp Biol Med. 1964 Oct;117:145–150. doi: 10.3181/00379727-117-29520. [DOI] [PubMed] [Google Scholar]
  9. SCOTT L. V., FELTON F. G., BARNEY J. A. Hemagglutination with herpes simplex virus. J Immunol. 1957 Mar;78(3):211–213. [PubMed] [Google Scholar]
  10. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  11. Sever J. L. Immunoglobulin determinations for the detection of perinatal infections. J Pediatr. 1969 Dec;75(6):1111–1115. doi: 10.1016/s0022-3476(69)80365-3. [DOI] [PubMed] [Google Scholar]
  12. Starr J. G., Calafiore D., Casey H. L. Experience with a human cytomegalovirus complement fixing antigen. Am J Epidemiol. 1967 Nov;86(3):507–512. doi: 10.1093/oxfordjournals.aje.a120761. [DOI] [PubMed] [Google Scholar]

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