Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1971 Jan;21(1):95–97. doi: 10.1128/am.21.1.95-97.1971

Reagent Strips and Conventional Tests for Acid Production from Mannitol and Coagulase Activity of Staphylococcus: a Comparative Study 1

Ernest J Anandam 1
PMCID: PMC377124  PMID: 4925001

Abstract

A total of 244 Staphylococcus strains were tested simultaneously for acid production from mannitol and for coagulase activity with reagent-impregnated paper strips and with their conventional counterparts. Significant correlation was obtained with 97.9% of the strains for mannitol and with 95% for the coagulase test. The paper strip method is a combined test for both mannitol and coagulase tests, thus making it more convenient and simpler than conventional methods. The results are obtained rapidly within 6 hr by the paper strip method. However, as the paper strip method is designed for the aerobic system, the conventional tests were also carried out under aerobic conditions to compare the results.

Full text

PDF
95

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EVANS J. B., NIVEN C. F., Jr A comparative study of known food-poisoning staphylococci and related varieties. J Bacteriol. 1950 Apr;59(4):545–550. doi: 10.1128/jb.59.4.545-550.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Evans J. B. Anaerobic Fermentation of Mannitol by Staphylococci. J Bacteriol. 1947 Aug;54(2):266–266. doi: 10.1128/jb.54.2.266-266.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Evans J. B. Studies of Staphylococci with Special Reference to the Coagulase-positive Types. J Bacteriol. 1948 Jun;55(6):793–800. doi: 10.1128/jb.55.6.793-800.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MOSSEL D. A., MARTIN G. [Simplified medium permitting study of the various modes of action of bacteria on carbohydrates]. Ann Inst Pasteur Lille. 1961;12:225–226. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES