Abstract
In an effort to obtain the flexibility and ease of performance of a rapid, serological test for detection of cytomegalovirus antibody, the indirect hemagglutination (IHA) technique was investigated by using a microserological system. Antigens were prepared from tissue cultures of infected human fibroblasts. The specificity of the cytomegalovirus antibody response detected by the IHA test correlated well with the standard neutralization test. The IHA method was more sensitive than the complement fixation test in detecting antibody in congenitally infected newborns. There appeared to be some heterologous antibody response with Herpesvirus hominis or varicella virus infections. The IHA test pattern was found to be very stable with excellent persistence of agglutination.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birnbaum G., Lynch J. I., Margileth A. M., Lonergan W. M., Sever J. L. Cytomegalovirus infections in newborn infants. J Pediatr. 1969 Nov;75(5):789–795. doi: 10.1016/s0022-3476(69)80301-x. [DOI] [PubMed] [Google Scholar]
- Fuccillo D. A., Catalano L. W., Jr, Moder F. L., Debus D. A., Sever J. L. Minicultures of mammalian cells in a new plastic plate. Appl Microbiol. 1969 Apr;17(4):619–622. doi: 10.1128/am.17.4.619-622.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fuccillo D. A., Moder F. L., Catalano L. W., Jr, Vincent M. M., Sever J. L. Herpesvirus hominis types I and II: a specific microindirect hemagglutination test. Proc Soc Exp Biol Med. 1970 Mar;133(3):735–739. doi: 10.3181/00379727-133-34554. [DOI] [PubMed] [Google Scholar]
- Lang D. J., Noren B. Cytomegaloviremia following congenital infection. J Pediatr. 1968 Dec;73(6):812–819. doi: 10.1016/s0022-3476(68)80233-1. [DOI] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- SEVER J. L., HUEBNER R. J., CASTELLANO G. A., BELL J. A. SEROLOGIC DIAGNOSIS "EN MASSE" WITH MULTIPLE ANTIGENS. Am Rev Respir Dis. 1963 Sep;88:SUPPL–359. doi: 10.1164/arrd.1963.88.3P2.342. [DOI] [PubMed] [Google Scholar]
- Stiehm E. R., Ammann A. J., Cherry J. D. Elevated cord macroglobulins in the diagnosis of intrauterine infections. N Engl J Med. 1966 Nov 3;275(18):971–977. doi: 10.1056/NEJM196611032751801. [DOI] [PubMed] [Google Scholar]
- WELLER T. H., HANSHAW J. B. Virologic and clinical observations on cytomegalic inclusion disease. N Engl J Med. 1962 Jun 14;266:1233–1244. doi: 10.1056/NEJM196206142662401. [DOI] [PubMed] [Google Scholar]