Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Jan;21(1):112–118. doi: 10.1128/am.21.1.112-118.1971

Rapid Method for the Isolation of Listeria monocytogenes from Experimentally Infected Mice 1

Thomas R Wilkinson 1,2, Elizabeth R Hall 1
PMCID: PMC377128  PMID: 4993226

Abstract

Listeria monocytogenes was successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr. A larger number of Listeria isolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr than from the other diluent suspensions. Holding infected tissues at 4 C for at least 3 months did not increase the efficiency of Listeria isolation. Listeria L-forms were not isolated from mice injected with the bacterial form. The in vitro viability of Listeria L-forms suspended in PBS or PBS splenic homogenate was greatly reduced when held at 4 C.

Full text

PDF
112

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avery R. J., Byrne J. L. An Attempt To Determine The Incidence Of Listeria Monocytogenes In The Brain Of Mammals. Can J Comp Med Vet Sci. 1959 Sep;23(9):296–300. [PMC free article] [PubMed] [Google Scholar]
  2. Edman D. C., Pollock M. B., Hall E. R. Listeria monocytogenes L forms. I. Induction maintenance, and biological characteristics. J Bacteriol. 1968 Aug;96(2):352–357. doi: 10.1128/jb.96.2.352-357.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GRAY M. L. Isolation of Listeria monocytogenes from oat silage. Science. 1960 Dec 9;132(3441):1767–1768. doi: 10.1126/science.132.3441.1767. [DOI] [PubMed] [Google Scholar]
  4. GRAY M. L., SMITH E. M., THORP F., Jr Reaction of splenic tissue in culture to Listeria monocytogenes. Proc Soc Exp Biol Med. 1957 Jan;94(1):162–166. doi: 10.3181/00379727-94-22886. [DOI] [PubMed] [Google Scholar]
  5. GRAY M. L., STAFSETH H. J., THORP F., Jr A four-year study of listeriosis in Michigan. J Am Vet Med Assoc. 1951 Apr;118(889):242–252. [PubMed] [Google Scholar]
  6. Gray M. L., Killinger A. H. Listeria monocytogenes and listeric infections. Bacteriol Rev. 1966 Jun;30(2):309–382. doi: 10.1128/br.30.2.309-382.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gray M. L., Stafseth H. J., Thorp F., Sholl L. B., Riley W. F. A New Technique for Isolating Listerellae from the Bovine Brain. J Bacteriol. 1948 Apr;55(4):471–476. [PMC free article] [PubMed] [Google Scholar]
  8. INHORN S. L., SMITS R. L., CHRISTENSON E. Listeria as a cause of splenic granulomas in a patient with Felty's syndrome. Am J Clin Pathol. 1960 Apr;33:330–338. doi: 10.1093/ajcp/33.4.330. [DOI] [PubMed] [Google Scholar]
  9. Kramer P. A., Jones D. Media selective for Listeria monocytogenes. J Appl Bacteriol. 1969 Sep;32(3):381–394. doi: 10.1111/j.1365-2672.1969.tb00987.x. [DOI] [PubMed] [Google Scholar]
  10. Louria D. B., Hensle T., Armstrong D., Collins H. S., Blevins A., Krugman D., Buse M. Listeriosis complicating malignant disease. A new association. Ann Intern Med. 1967 Aug;67(2):260–281. doi: 10.7326/0003-4819-67-2-261. [DOI] [PubMed] [Google Scholar]
  11. OLSON C., Jr, DUNN L. A., ROLLINS C. L. Methods for isolation of Listeria monocytogenes from sheep. Am J Vet Res. 1953 Jan;14(50):82–85. [PubMed] [Google Scholar]
  12. OSEBOLD J. W., INOUYE T. Pathogenesis of Listeria monocytogenes infections in natural hosts. II. Sheep studies. J Infect Dis. 1954 Jul-Aug;95(1):67–78. doi: 10.1093/infdis/95.1.67. [DOI] [PubMed] [Google Scholar]
  13. OSEBOLD J. W., KENDRICK J. W., NJOKU-OBI A. Abortion of cattle experimentally with Listeria monocytogenes. J Am Vet Med Assoc. 1960 Aug 15;137:227–233. [PubMed] [Google Scholar]
  14. Pittman B., Cherry W. B. Isolation of Listeria monocytogenes from brains of rabies-negative animals. Am J Vet Res. 1967 May;28(124):779–785. [PubMed] [Google Scholar]
  15. RAPPAPORT F., RABINOVITZ M., TOAFF R., KROCHIK N. Genital listeriosis as a cause of repeated abortion. Lancet. 1960 Jun 11;1(7137):1273–1275. doi: 10.1016/s0140-6736(60)92253-4. [DOI] [PubMed] [Google Scholar]
  16. Welshimer H. J. Isolation of Listeria monocytogenes from vegetation. J Bacteriol. 1968 Feb;95(2):300–303. doi: 10.1128/jb.95.2.300-303.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wilkinson T. R., Hall E. R. Survival of Listeria monocytogenes in experimentally infected mice. Appl Microbiol. 1971 Jan;21(1):108–111. doi: 10.1128/am.21.1.108-111.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. ZINK A., de MELLO G. C., BURKHART R. L. Listeriosis; field and laboratory studies, and aureomycin activity. Am J Vet Res. 1951 Jul;12(44):194–198. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES