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. 1971 Jan;21(1):112–118. doi: 10.1128/am.21.1.112-118.1971

Rapid Method for the Isolation of Listeria monocytogenes from Experimentally Infected Mice 1

Thomas R Wilkinson 1,2, Elizabeth R Hall 1
PMCID: PMC377128  PMID: 4993226

Abstract

Listeria monocytogenes was successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr. A larger number of Listeria isolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr than from the other diluent suspensions. Holding infected tissues at 4 C for at least 3 months did not increase the efficiency of Listeria isolation. Listeria L-forms were not isolated from mice injected with the bacterial form. The in vitro viability of Listeria L-forms suspended in PBS or PBS splenic homogenate was greatly reduced when held at 4 C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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