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. 1971 Jan;21(1):124–131. doi: 10.1128/am.21.1.124-131.1971

Morphological Alterations in Bacillus megaterium as Produced by Aflatoxin B11

L R Beuchat 1,2, R V Lechowich 1
PMCID: PMC377130  PMID: 4993228

Abstract

Morphologically abnormal cells were produced by Bacillus megaterium NRRL B-1368 in response to aflatoxin B1. Filamentous forms were characterized by early granulation and unusually large and numerous deposits of poly-β-hydroxybutyric acid within the cells. Pantoyl lactone was without effect as a reversing agent for the observed inhibition of cell septum formation. B. megaterium cells and spores produced on toxic (3.8 μg of aflatoxin B1 per ml) and nontoxic Trypticase Soy Broth and Trypticase Soy Agar (TSA) were observed by using phase contrast and electron microscopy. Transfer of aberrant forms to nontoxic TSA yielded macrocolonies with daughter cells morphologically indistinguishable from untreated cells. Agar slide cultures of filamentous cells transferred to nontoxic TSA indicated that normal cells were formed. Electron photomicrographs showed a decreased number of mesosomes in filamentous cells as compared to control cells. There were no observable morphological differences in spores formed on toxic or nontoxic TSA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beuchat L. R., Lechowich R. V. Biochemical alterations in Bacillus megaterium as produced by aflatoxin B1. Appl Microbiol. 1971 Jan;21(1):119–123. doi: 10.1128/am.21.1.119-123.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clifford J. I., Rees K. R. The interaction of aflatoxins with purines and purine nucleosides. Biochem J. 1967 May;103(2):467–471. doi: 10.1042/bj1030467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Freer J. H., Levinson H. S. Fine structure of Bacillus megaterium during microcycle sporogenesis. J Bacteriol. 1967 Aug;94(2):441–457. doi: 10.1128/jb.94.2.441-457.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. GRULA M. M., GRULA E. A. Cell division in a species of Erwinia IV. Metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division. J Bacteriol. 1962 May;83:989–997. doi: 10.1128/jb.83.5.989-997.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gabliks J., Schaeffer W., Friedman L., Wogan G. Effect of Aflatoxin B(1) on Cell Cultures. J Bacteriol. 1965 Sep;90(3):720–723. doi: 10.1128/jb.90.3.720-723.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lillehoj E. B., Ciegler A. Aflatoxin B1 binding and toxic effects on Bacillus megaterium. J Gen Microbiol. 1968 Dec;54(2):185–194. doi: 10.1099/00221287-54-2-185. [DOI] [PubMed] [Google Scholar]
  8. Lillehoj E. B., Ciegler A., Hall H. H. Aflatoxin B 1 uptake by Flavobacterium aurantiacum and resulting toxic effects. J Bacteriol. 1967 Jan;93(1):464–471. doi: 10.1128/jb.93.1.464-471.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sporn M. B., Dingman C. W., Phelps H. L., Wogan G. N. Aflatoxin B1: binding to DNA in vitro and alteration of RNA metabolism in vivo. Science. 1966 Mar 25;151(3717):1539–1541. doi: 10.1126/science.151.3717.1539. [DOI] [PubMed] [Google Scholar]

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