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(A) Liquid chromatography indicates a single uniformly modified chemical species at the expected mass/charge (m/z) ratio, and only 2% of combined signal is seen from the expected m/z peak for the unmodified peptide. (B) MS/MS spectrum of the derivatized H4K16ac protein PropionylGKpropionylGGKpropionylGLGKpropionylGGAKAcetylR. MS/MS Spectrum with b (N-terminal) and y (C-terminal) fragment ions labeled. Best manual fragmentation pattern match assigns acetylation to K16.
