Abstract
Heterotrophic conversion of l-aspartic acid to nitrification products by Aspergillus flavus was studied in a replacement incubation system. Numerous amino acids supported nitrification; aspartate and glutamate were about equivalent as the best sources of nitrate. Addition of sodium bicarbonate to the incubation system substantially enhanced nitrate formation for all nitrifiable amino acids except aspartic acid, but the basis for the bicarbonate effect is obscure. The yield of nitrate from l-aspartate was not approached by forms of aspartic acid resulting from substitution on the beta carbon, the amino nitrogen, or the gamma carboxyl group or by aspartate presented as the d-configuration. There was no relationship between nitrate formation and the occurrence of such possible intermediates as nitrite, bound hydroxylamine, ammonia, aspergillic acid, and beta-nitropropionic acid. Uniformly labeled 14C-l-aspartate that was nitrified in replacement incubation led to no accumulation of label in possible nitrification products in the culture filtrate. Label was found in components of the mycelium after acid hydrolysis, with heaviest accumulation in what appeared to be glucosamine and an unidentified compound, possibly acetylglucosamine. Detectable label was redistributed into serine, glycine, and threonine.
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- ALEEM M. I., LEES H., LYRIC R. AMMONIUM OXIDATION BY CELL-FREE EXTRACTS OF ASPERGILLUS WENTII. Can J Biochem. 1964 Jul;42:989–998. doi: 10.1139/o64-109. [DOI] [PubMed] [Google Scholar]
- BECKER G. E., SCHMIDT E. L. BETA-NITROPROPIONIC ACID AND NITRITE IN RELATION TO NITRATE FORMATION BY ASPERGILLUS FLAVUS. Arch Mikrobiol. 1964 Aug 17;49:165–175. [PubMed] [Google Scholar]
- BUSH M. T., TOUSTER O., BROCKMAN J. E. The production of beta-nitropropionic acid by a strain of Aspergillus flavus. J Biol Chem. 1951 Feb;188(2):685–693. [PubMed] [Google Scholar]
- Doxtader K. G., Alexander M. Nitrification by growing and replacement cultures of Aspergillus. Can J Microbiol. 1966 Aug;12(4):807–815. doi: 10.1139/m66-109. [DOI] [PubMed] [Google Scholar]
- Doxtader K. G., Alexander M. Role of 3-Nitropropanoic acid in nitrate formation by Aspergillus flavus. J Bacteriol. 1966 Mar;91(3):1186–1191. doi: 10.1128/jb.91.3.1186-1191.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EYLAR O. R., Jr, SCHMIDT E. L. A survey of heterotrophic micro-organisms from soil for ability to form nitrite and nitrate. J Gen Microbiol. 1959 Jun;20(3):473–481. doi: 10.1099/00221287-20-3-473. [DOI] [PubMed] [Google Scholar]
- Marshall K. C. The role of beta-alanine in the biosynthesis of nitrate by Aspergillus flavus. Antonie Van Leeuwenhoek. 1965;31(4):386–394. doi: 10.1007/BF02045918. [DOI] [PubMed] [Google Scholar]
- Novak R., Wilson P. W. The Utilization of Nitrogen in Hydroxylamine and Oximes by Azotobacter vinelandii. J Bacteriol. 1948 Apr;55(4):517–524. doi: 10.1128/jb.55.4.517-524.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHMIDT E. L. Nitrate formation by a soil fungus. Science. 1954 Feb 5;119(3084):187–189. doi: 10.1126/science.119.3084.187. [DOI] [PubMed] [Google Scholar]
- Shaw P. D., DeAngelo A. B. Role of ammonium ion in the biosynthesis of beta-nitropropionic acid. J Bacteriol. 1969 Aug;99(2):463–468. doi: 10.1128/jb.99.2.463-468.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw P. D., McCloskey J. A. Biosynthesis of nitro compounds. II. Studies on potential precursors for the nitro group of beta-nitropropionic acid. Biochemistry. 1967 Jul;6(7):2247–2260. doi: 10.1021/bi00859a047. [DOI] [PubMed] [Google Scholar]