Abstract
The use of a cotton gauze swab and subsequent culture of the swab was found to be a more sensitive method for isolating Salmonella from liquid milk than the revised procedure of North. The swab method was found to be as sensitive as the North procedure for recovering Salmonella when incubated at 37 C but more sensitive when incubated at 43 C. Incubation of the swab cultures at the elevated temperature of 43 C gave good results when Salmonella was present at levels as low as one per liter. Swabs exposed to milk contaminated with 100 Salmonella per liter remained positive even when subsequently washed for 2 hr in noncontaminated milk. Bismuth sulfite agar and Brilliant Green sulfadiazine agar were equally effective for isolating Salmonella from broth cultures; use of both media resulted in maximal isolations.
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Selected References
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- Carlson V. L., Snoeyenbos G. H., McKie B. A., Smyser C. F. A comparison of incubation time and temperature for the isolation of salmonella. Avian Dis. 1967 May;11(2):217–225. [PubMed] [Google Scholar]
- Collins R. N., Treger M. D., Goldsby J. B., Boring J. R., 3rd, Coohon D. B., Barr R. N. Interstate outbreak of Salmonella newbrunswick infection traced to powdered milk. JAMA. 1968 Mar 4;203(10):838–844. [PubMed] [Google Scholar]
- Harvey R. W., Price T. H. Elevated temperature incubation of enrichment media for the isolation of salmonellas from heavily contaminated materials. J Hyg (Lond) 1968 Sep;66(3):377–381. doi: 10.1017/s0022172400041243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JAMESON J. E. A study of tetrathionate enrichment techniques, with particular reference to two new tetrathionate modifications used in isolating salmonellae from sewer swabs. J Hyg (Lond) 1961 Mar;59:1–13. doi: 10.1017/s0022172400038663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Julseth R. M., Deibel R. H. Effect of temperature on growth of Salmonella in rehydrated skim milk from a food-poisoning outbreak. Appl Microbiol. 1969 May;17(5):767–768. doi: 10.1128/am.17.5.767-768.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris G. K., Dunn C. G. Influence of incubation temperature and sodium heptadecyl sulfate (Tergitol No. 7) on the isolation of salmonellae from pork sausage. Appl Microbiol. 1970 Aug;20(2):192–195. doi: 10.1128/am.20.2.192-195.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris G. K., Martin W. T., Shelton W. H., Wells J. G., Brachman P. S. Salmonellae in fish meal plants: relative amounts of contamination at various stages of processing and a method of control. Appl Microbiol. 1970 Mar;19(3):401–408. doi: 10.1128/am.19.3.401-408.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTH W. R., Jr Use of crystal violet or brilliant green dyes for the determination of Salmonellae in dried food products. J Bacteriol. 1960 Dec;80:861–861. doi: 10.1128/jb.80.6.861-861.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]