Abstract
A quantitative micro-complement fixation test capable of detecting nanogram quantities of antigen is described. The test is simple to perform and is highly reproducible. Typical results from three antigen-antibody systems are given.
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- GERBER P., BIRCH S. M. ANTIGENIC ANALYSIS OF VIRULENT AND ATTENUATED TYPE I POLIOVIRUSES BY QUANTITATIVE COMPLEMENT FIXATION. J Immunol. 1965 Jul;95:70–74. [PubMed] [Google Scholar]
- GERSTEIN J. F., LEVINE L., VANVUNAKIS H. ALTERED ANTIGENICITY OF PEPSINOGEN AND PEPSIN AS AN INDEX OF CONFORMATIONAL CHANGE: EFFECT OF UREA AND REDUCING AGENTS. Immunochemistry. 1964 Apr;1:3–14. doi: 10.1016/0019-2791(64)90050-3. [DOI] [PubMed] [Google Scholar]
- Moore B. W., Perez V. J. Complement fixation for antigens on a picogram level. J Immunol. 1966 Jun;96(6):1000–1005. [PubMed] [Google Scholar]
- Murphy T. M., Mills S. E. Immunochemical comparisons of mutant and wild-type alpha-subunits of tryptophan synthetase. Arch Biochem Biophys. 1968 Sep 20;127(1):7–16. doi: 10.1016/0003-9861(68)90194-x. [DOI] [PubMed] [Google Scholar]
- Sarich V. M., Wilson A. C. Quantitative immunochemistry and the evolution of primate albumins: micro-complement fixation. Science. 1966 Dec 23;154(3756):1563–1566. doi: 10.1126/science.154.3756.1563. [DOI] [PubMed] [Google Scholar]
- WASSERMAN E., LEVINE L. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961 Sep;87:290–295. [PubMed] [Google Scholar]