Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Feb;21(2):300–305. doi: 10.1128/am.21.2.300-305.1971

Renal Lysozyme Levels in Animals Developing Proteus mirabilis-Induced Pyelonephritis

W W Eudy 1, S E Burrous 1
PMCID: PMC377165  PMID: 5549703

Abstract

In animals developing experimentally induced unilateral pyelonephritis, both the infected kidney (IK) and the contralateral noninfected kidney (NIK) showed an immediate increase in renal lysozyme activity of about 5 days' duration after the unilateral injection of viable Proteus mirabilis into the renal cortex. Lysozyme activities of the NIK were consistently higher than those of the IK. This initial increase was followed by a second increase which lasted throughout the period of observation (17 days), and enzyme activities of the NIK were consistently higher than those of the IK. In saline punctured kidneys of control animals, both the saline punctured kidney (SP) and the non-saline punctured kidney (NSP) showed only the immediate increase in renal lysozyme activity, which persisted until the SP was completely healed. These enzyme activities were less than those observed in the infected animals, but the response of the NSP was greater than that of the SP. Trauma not directed to the kidney does not produce a similar response of renal lysozyme. The elevated renal lysozyme of the NIK could not be shown to protect it from bacterial infection.

Full text

PDF
300

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burrous S. E., Cawein J. B. Rat pyelonephritis model suitable for primary or secondary screening. Appl Microbiol. 1969 Sep;18(3):448–451. doi: 10.1128/am.18.3.448-451.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gault M. H., Geggie P. H. Clinical significance of urinary LDH, alkaline phosphatase and other enzymes. Can Med Assoc J. 1969 Aug 23;101(4):208–215. [PMC free article] [PubMed] [Google Scholar]
  3. Goldberg W. M., Chakrabarti S., Filipich R. Urinary lactic dehydrogenase, alkaline phosphatase and lysozyme studies in renal disease. Can Med Assoc J. 1966 Jun 11;94(24):1264–1268. [PMC free article] [PubMed] [Google Scholar]
  4. Hamdy M. K. Effect of trauma and infection on lyozyme in poultry tissue. Proc Soc Exp Biol Med. 1969 Jun;131(2):409–415. doi: 10.3181/00379727-131-33890. [DOI] [PubMed] [Google Scholar]
  5. Hayslett J. P., Perillie P. E., Finch S. C. Urinary muramidase and renal disease. Correlation with renal histology and implication for the mechanism of enzymuria. N Engl J Med. 1968 Sep 5;279(10):506–512. doi: 10.1056/NEJM196809052791002. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Lingårdh G., Andersson L., Voigt A., Olin T. Urinary enzyme studies following renal ischaemia in dogs. Scand J Urol Nephrol. 1968;2(3):165–172. doi: 10.3109/00365596809135362. [DOI] [PubMed] [Google Scholar]
  8. Miller T. E., Cameron C. M., North J. D. Distribution of lysozyme in the rat kidney and the role of this enzyme in experimental pyelonephritis. Proc Soc Exp Biol Med. 1968 Jul;128(3):749–752. doi: 10.3181/00379727-128-33115. [DOI] [PubMed] [Google Scholar]
  9. Noble R. E., Najarian J. S., Brainerd H. D. Urine and serum lysozyme measurement in renal homotransplantation. Proc Soc Exp Biol Med. 1965 Dec;120(3):737–740. doi: 10.3181/00379727-120-30641. [DOI] [PubMed] [Google Scholar]
  10. PERRI G. C., CAPPUCCINO J. G., FAULK M., MELLORS J., STOCK C. C. Variations of the content of lysozyme in normal rats and rats bearing Jensen sarcoma following surgery. Cancer Res. 1963 Mar;23:431–435. [PubMed] [Google Scholar]
  11. PERRI G. C., FAULK M., SHAPIRO E., MONEY W. L. ROLE OF THE KIDNEY IN ACCUMULATION OF EGG WHITE MURAMIDASE IN EXPERIMENTAL ANIMALS. Proc Soc Exp Biol Med. 1964 Jan;115:189–192. doi: 10.3181/00379727-115-28866. [DOI] [PubMed] [Google Scholar]
  12. PROCKOP D. J., DAVIDSON W. D. A STUDY OF URINARY AND SERUM LYSOZYME IN PATIENTS WITH RENAL DISEASE. N Engl J Med. 1964 Feb 6;270:269–274. doi: 10.1056/NEJM196402062700602. [DOI] [PubMed] [Google Scholar]
  13. Pelligrini R., Vertova P. Synergism between penicillins and lysozyme. Data which led to the synthesis of a new antibiotic salt: lysozyme ampicillinate. 3. Arzneimittelforschung. 1969 Mar;19(3):375–379. [PubMed] [Google Scholar]
  14. Sussman M., Asscher A. W., Jenkins J. A. The intrarenal distribution of lysozyme (muramidase). Invest Urol. 1968 Sep;6(2):148–152. [PubMed] [Google Scholar]
  15. Wilkinson J. H. Diagnostic significance of enzyme determinations in urine. Curr Probl Clin Biochem. 1968;2(0):207–227. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES