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. 1971 Feb;21(2):311–315. doi: 10.1128/am.21.2.311-315.1971

Statistical Evaluation of Diluents and Automatic Diluting and Pipetting Machines in Influenza Serology

Thomas C O'Brien 1, Suresh Rastogi 1, Nicola M Tauraso 1
PMCID: PMC377167  PMID: 5549704

Abstract

The use of three diluents (i.e., 0.01 m phosphate-buffered saline, PBS; PBS with 0.2% gelatin, PBS/GEL; and PBS with 0.4% bovine plasma albumin) and three methods (i.e., the standard tube macro-procedure, TUBE; the manual microtechnique, MANUAL; and the semiautomatic microtechnique, AUTO) were statistically compared for their reproducibility and sensitivity in determining hemagglutinin (HA) and hemagglutination-inhibition (HI) antibody titers. In the HA test, analyses of between-cell variances of the different methods showed the AUTO microtiter procedure to be more reproducible than the standard TUBE method. The MANUAL microtiter procedure was the least reproducible. In the HI test, the TUBE method was the most reproducible. No significant difference in the reproducibility of the diluents was observed in either the HA or HI test. When a comparison of the sensitivity of test methods and diluents was made for determining HA titers, the AUTO microtiter procedure and PBS/GEL diluent appeared to be the method and diluent of choice. Evaluation of another instrument, the autopipetter, which standardizes the volume of diluent to be added in the microtechnique, suggests that the reproducibility of the AUTO microtiter procedure might be further increased.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hierholzer J. C., Suggs M. T. Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions. Appl Microbiol. 1969 Nov;18(5):816–823. doi: 10.1128/am.18.5.816-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hirata A. A., Grant D. S., Draper L. R. Factors affecting the passive hemagglutination titration: dilution loops, titration trays, vibration, diluents. Appl Microbiol. 1969 Apr;17(4):563–567. doi: 10.1128/am.17.4.563-567.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Leiderman E., Mogabgab W. J. Antigenicity of influenza vaccine from bovine cell cultures. Appl Microbiol. 1969 Oct;18(4):596–600. doi: 10.1128/am.18.4.596-600.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MacLowry J. D., Marsh H. H. Semiautomatic microtechnique for serial dilution-antibiotic sensitivity testing in the clinical laboratory. J Lab Clin Med. 1968 Oct;72(4):685–687. [PubMed] [Google Scholar]
  6. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  7. Tauraso N. M., Gleckman R., Pedreira F. A., Sabbaj J., Yahwak R., Madoff M. A. Effect of dosage and route of inoculation upon antigenicity of inactivated influenza virus vaccine (Hong Kong strain) in man. Bull World Health Organ. 1969;41(3):507–516. [PMC free article] [PubMed] [Google Scholar]

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