Abstract
Differentiation of Salmonella from other gram-negative bacilli requires several biochemical and serological tests. A simplified 24-hr screening procedure has been devised which allows discarding of large numbers of isolates (picked from selective plating media) before they are subjected to this extensive testing. Cultures of gram-negative organisms isolated to triple sugar-iron slants during routine examination of products for Salmonella were tested for the presence of β-galactosidase and Salmonella flagellar antigens. β-Galactosidase-positive cultures which did not agglutinate in polyvalent flagellar antiserum were considered to be nonsalmonellae. Of 1,103 Salmonella cultures tested, none of the 61 different serotypes was missed by this procedure, whereas 673 (82.3%) of 818 nonsalmonellae were excluded from further testing. This screening procedure eliminates most nonsalmonellae and augments the proportion of cultures undergoing further biochemical and serological testing which will be confirmed as Salmonella.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BUELOW P. THE ONPG TEST IN DIAGNOSTIC BACTERIOLOGY. COMPARISON OF THE ONPG TEST AND THE CONVENTIONAL LACTOSE-FERMENTATION TEST. Acta Pathol Microbiol Scand. 1964;60:387–402. doi: 10.1111/apm.1964.60.3.387. [DOI] [PubMed] [Google Scholar]
- Johnston M. A., Thatcher F. S. Selective inhibition of Escherichia coli in the presence of Salmonella typhimurium by phenethyl beta-D-galactopyranoside. Appl Microbiol. 1967 Sep;15(5):1223–1227. doi: 10.1128/am.15.5.1223-1228.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAPAGE S. P., JAYARAMAN M. S. BETA-GALACTOSIDASE AND LACTOSE FERMENTATION IN THE IDENTIFICATION OF ENTEROBACTERIA INCLUDING SALMONELLAE. J Clin Pathol. 1964 Mar;17:117–121. doi: 10.1136/jcp.17.2.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEDERBERG J. The beta-d-galactosidase of Escherichia coli, strain K-12. J Bacteriol. 1950 Oct;60(4):381–392. doi: 10.1128/jb.60.4.381-392.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pickett M. J., Goodman R. E. Beta-galactosidase for distinguishing between Citrobacter and Salmonella. Appl Microbiol. 1966 Mar;14(2):178–182. doi: 10.1128/am.14.2.178-182.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]