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. 1971 Feb;21(2):350–357. doi: 10.1128/am.21.2.350-357.1971

Biological Characteristics of Cloned Populations of Herpes Simplex Virus Types 1 and 2

Jerry W Smith 1,1, José E Rodriguez 1, Albert P McKee 1,2
PMCID: PMC377175  PMID: 5544298

Abstract

By using cloned types 1 and 2 herpes simplex virus, obtained by selecting large and small plaques produced by material from human lesions, studies were performed to compare properties between preparations of each type. Regarding the rate of inactivation by ultraviolet light, no differences were found between the two antigenic types and none between the preparations obtained from either type. In contrast, type 1 preparations were found to be more readily inactivated at 45 C than type 2. Plaque size of cloned preparations changed by passage in cell culture. A broader range of plaque sizes was obtained, and average plaque size was larger. After 20 passages, preparations obtained from different types gave rise to one of three kinds of cytopathic effect. The cytopathic effect produced by type 1 preparations remained as before 20 passages and consisted of round cells in a compact central mass. For type 2, two kinds of cytopathic effect were seen in cloned preparations. This consisted of aggregates of round cells (seen in preparations before 20 passages) or of large, loose aggregates of round cells of various sizes. Results from neutralization studies using virus before and after 20 passages in cell culture versus antisera prepared against live or ultraviolet-inactivated virus showed no differences between cloned preparations obtained from a given type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHE W. K., SCHERP H. W. ANTIGENIC ANALYSIS OF HERPES SIMPLEX VIRUS BY NEUTRALIZATION KINETICS. J Immunol. 1963 Nov;91:658–665. [PubMed] [Google Scholar]
  2. Couch E. F., Nahmias A. J. Filamentous structures of type 2 Herpesvirus hominis infection of the chorioallantoic membrane. J Virol. 1969 Feb;3(2):228–232. doi: 10.1128/jvi.3.2.228-232.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dowdle W. R., Nahmias A. J., Harwell R. W., Pauls F. P. Association of antigenic type of Herpesvirus hominis with site of viral recovery. J Immunol. 1967 Nov;99(5):974–980. [PubMed] [Google Scholar]
  4. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  5. Figueroa M. E., Rawls W. E. Biological markers for differentiation of herpes-virus strains of oral and genital origin. J Gen Virol. 1969 Mar;4(2):259–267. doi: 10.1099/0022-1317-4-2-259. [DOI] [PubMed] [Google Scholar]
  6. GRAY A., TOKUMARU T., SCOTT T. F. M. Different cytopathogenic effects observed in HeLa cells infected with herpes simplex virus. Arch Gesamte Virusforsch. 1958;8(1):59–76. doi: 10.1007/BF01242313. [DOI] [PubMed] [Google Scholar]
  7. Germanov A. B., Sokolov M. I. Selection of herpesvirus mutants producing mottled plaques in human fibroblast monolayers. (Brief report). Arch Gesamte Virusforsch. 1969;28(1):85–88. doi: 10.1007/BF01250849. [DOI] [PubMed] [Google Scholar]
  8. Goodheart C. R., Plummer G., Waner J. L. Density difference of DNA of human herpes simplex viruses, types I and II. Virology. 1968 Jul;35(3):473–475. doi: 10.1016/0042-6822(68)90225-0. [DOI] [PubMed] [Google Scholar]
  9. HINZE H. C., WALKER D. L. Variation of herpes simplex virus in persistently infected tissue cultures. J Bacteriol. 1961 Oct;82:498–504. doi: 10.1128/jb.82.4.498-504.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HOGGAN M. D., ROIZMAN B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg. 1959 Sep;70:208–219. doi: 10.1093/oxfordjournals.aje.a120071. [DOI] [PubMed] [Google Scholar]
  11. Hampar B. Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody. J Bacteriol. 1966 Dec;92(6):1741–1747. doi: 10.1128/jb.92.6.1741-1747.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KOHLHAGE H., SIEGERT R. [2 genetically determined variants of a herpes simplex strain]. Arch Gesamte Virusforsch. 1962;12:273–286. [PubMed] [Google Scholar]
  13. Murphy F. A., Harrison A. K., Whitfield S. G. Intranuclear formation of filaments in herpesvirus hominis infection of mice. Arch Gesamte Virusforsch. 1967;21(3):463–468. doi: 10.1007/BF01241746. [DOI] [PubMed] [Google Scholar]
  14. NII S., KAMAHORA J. Cytopathic changes induced by herpes simplex virus. Biken J. 1961 Dec;4:255–270. [PubMed] [Google Scholar]
  15. Nahmias A. J., Dowdle W. R. Antigenic and biologic differences in herpesvirus hominis. Prog Med Virol. 1968;10:110–159. [PubMed] [Google Scholar]
  16. Nahmias A. J., Dowdle W. R., Naib Z. M., Highsmith A., Harwell R. W., Josey W. E. Relation of pock size on chorioallantoic membrane to antigenic type of herpesvirus hominis. Proc Soc Exp Biol Med. 1968 Apr;127(4):1022–1028. doi: 10.3181/00379727-127-32861. [DOI] [PubMed] [Google Scholar]
  17. Parker J. D., Banatvala J. E. Herpes genitalis; clinical and virological studies. Br J Vener Dis. 1967 Sep;43(3):212–216. doi: 10.1136/sti.43.3.212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Plummer G., Waner J. L., Bowling C. P. Comparative studies of type 1 and type 2 & 'herpes simplex' viruses. Br J Exp Pathol. 1968 Apr;49(2):202–208. [PMC free article] [PubMed] [Google Scholar]
  19. Plummer G., Waner J. L., Phuangsab A., Goodheart C. R. Type 1 and type 2 herpes simplex viruses: serological and biological differences. J Virol. 1970 Jan;5(1):51–59. doi: 10.1128/jvi.5.1.51-59.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. RAPP F. VARIANTS OF HERPES SIMPLEX VIRUS: ISOLATION, CHARACTERIZATION, AND FACTORS INFLUENCING PLAQUE FORMATION. J Bacteriol. 1963 Nov;86:985–991. doi: 10.1128/jb.86.5.985-991.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WHEELER C. E. Herpes simplex virus. Characteristics of a strain which produces unusually large multinucleated giat cells in tissue culture. Arch Dermatol. 1960 Sep;82:391–399. doi: 10.1001/archderm.1960.01580030085011. [DOI] [PubMed] [Google Scholar]
  22. Wallis C., Melnick J. L. Thermostabilization and thermosensitization of herpesvirus. J Bacteriol. 1965 Dec;90(6):1632–1637. doi: 10.1128/jb.90.6.1632-1637.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wheeler C. E., Jr, Briggaman R. A., Henderson R. R. Discrimination between two strains (types) of Herpes simplex virus by various modifications of the neutralization test. J Immunol. 1969 May;102(5):1179–1192. [PubMed] [Google Scholar]

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