Abstract
Mumps virus was grown in embryonated chicken eggs in the presence of radioactive seleno-(75Se)-methionine. Virus in the allantoic and amniotic fluids was concentrated in a sucrose density gradient, and a peak of viral material coincided with a significant peak of 75Se-radioactivity. The radioactivity was acid-insoluble and remained associated with the virus after purification by erythrocyte adsorption and elution and centrifugation on a second sucrose density gradient. After amino-acid hydrolysis of the radioactive virus, only 75Se-methionine was recovered by chromatographic analysis. These results demonstrate that the radioactive 75Se-methionine was incorporated into protein of infectious mumps virus.
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Selected References
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