Abstract
Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 μg/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.
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- Cuatrecasas P., Fuchs S., Anfinsen C. B. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus. J Biol Chem. 1967 Apr 10;242(7):1541–1547. [PubMed] [Google Scholar]
- LERMAN L. S. The structure of the DNA-acridine complex. Proc Natl Acad Sci U S A. 1963 Jan 15;49:94–102. doi: 10.1073/pnas.49.1.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachica R. V., Deibel R. H. Detection of nuclease activity in semisolid and broth cultures. Appl Microbiol. 1969 Aug;18(2):174–176. doi: 10.1128/am.18.2.174-176.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STREITFELD M. M., HOFFMANN E. M., JANKLOW H. M. Evaluation of extracellular deoxyribonuclease activity in Pseudomonas. J Bacteriol. 1962 Jul;84:77–80. doi: 10.1128/jb.84.1.77-80.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Victor R., Lachica F., Weiss K. F., Deibel R. H. Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus. Appl Microbiol. 1969 Jul;18(1):126–127. doi: 10.1128/am.18.1.126-127.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]