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. 1971 Apr;21(4):588–593. doi: 10.1128/am.21.4.588-593.1971

Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1

Gerald W Strandberg 1, Karl L Smiley 1
PMCID: PMC377236  PMID: 5575565

Abstract

Properties were determined of the glucose isomerase from Streptomyces phaeochromogenes NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a pH optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum pH were studied. Michaelis constants for glucose and Mg2+ were 0.25 and 0.025 m, respectively. Co2+ enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.

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Selected References

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