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. 1971 Apr;21(4):594–599. doi: 10.1128/am.21.4.594-599.1971

Identification of Mycoplasmatales: Procedures for Both Characterization and Purification 1

Thomas L Barber 1,2, Julius Fabricant 1
PMCID: PMC377237  PMID: 4930273

Abstract

Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. Two of 11 avian serotypes were resistant to 0.02% methylene blue. A number of Mycoplasma strains grew at 42 C or in a pH 9.5 medium. Usefulness of these procedures for purifying cultures of Mycoplasma is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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