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. 1971 Apr;21(4):639–642. doi: 10.1128/am.21.4.639-642.1971

Tetracycline Inhibition of a Lipase from Corynebacterium acnes

K Weaber 1, R Freedman 1, W W Eudy 1
PMCID: PMC377246  PMID: 4252558

Abstract

A lipase which hydrolyzes triglycerides (tricaprylin and trilaurin) and naphthyl laurate was obtained from the broth of Corynebacterium acnes cultures by ammonium sulfate fractionation. Ca2+ and sodium taurocholate stimulated activity of the enzyme. Ethylenediaminetetraacetic acid (EDTA) did not inhibit activity of the Ca2+-activated enzyme, but lipolytic activity was inhibited by EDTA in the absence of Ca2+. Tetracycline (10−4m) produced a slight inhibition of the lipase activity with 5 × 10−5m or less showing no effect on the lipase activity. However, complete inhibition by tetracycline at 10−4m was observed for Ca2+-activated enzyme. Tetracycline inhibition of the C. acnes lipase could be demonstrated at concentrations as low as 10−6m.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Faget H., Landes E. Untersuchungen über die Wirkung der Tetracycline bei der Acne vulgaris. Hautarzt. 1968 Oct;19(10):469–472. [PubMed] [Google Scholar]
  2. Freinkel R. K., Strauss J. S., Yip S. Y., Pochi P. E. Effect of tetracycline on the composition of sebum in acne vulgaris. N Engl J Med. 1965 Oct 14;273(16):850–854. doi: 10.1056/NEJM196510142731604. [DOI] [PubMed] [Google Scholar]
  3. ISHIDATE M., SAKAGUCHI T. Metal chelate compounds of tetracycline derivatives. I. Aureomycin. Pharm Bull. 1955 Apr;3(2):147–155. doi: 10.1248/cpb1953.3.147. [DOI] [PubMed] [Google Scholar]
  4. Kellum R. E., Strangfeld K., Ray L. F. Acne vulgaris. Studies in pathogenesis: triglyceride hydrolysis by Corynebacterium acnes in vitro. Arch Dermatol. 1970 Jan;101(1):41–47. doi: 10.1001/archderm.101.1.41. [DOI] [PubMed] [Google Scholar]
  5. Kellum R. E., Strangfeld K. Triglyceride hydrolysis by Corynebacterium acnes in vitro. J Invest Dermatol. 1969 Mar;52(3):255–258. [PubMed] [Google Scholar]
  6. NICOLAIDES N., WELLS G. C. On the biogenesis of the free fatty acids in human skin surface fat. J Invest Dermatol. 1957 Dec;29(6):423–433. doi: 10.1038/jid.1957.118. [DOI] [PubMed] [Google Scholar]
  7. Oterholm A., Ordal Z. J., Witter L. D. Purification and properties of a glycerol ester hydrolase (lipase) from Propionibacterium shermanii. Appl Microbiol. 1970 Jul;20(1):16–22. doi: 10.1128/am.20.1.16-22.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Reisner R. M., Puhvel M. Lipolytic activity of Staphylococcus albus. J Invest Dermatol. 1969 Jul;53(1):1–7. [PubMed] [Google Scholar]
  9. SELIGMAN A. M., NACHLAS M. M. The colorimetric determination of lipase and esterase in human serum. J Clin Invest. 1950 Jan;29(1):31–36. doi: 10.1172/JCI102231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. STRAUSS J. S., MESCON H. The chemical determination of special lipases in comedones. J Invest Dermatol. 1959 Oct;33:191–192. [PubMed] [Google Scholar]
  11. Shalita A. R., Wheatley V. Inhibition of pancreatic lipase by tetracyclines. J Invest Dermatol. 1970 May;54(5):413–415. doi: 10.1111/1523-1747.ep12259225. [DOI] [PubMed] [Google Scholar]
  12. Strauss J. S., Pochi P. E. Intracutaneous injection of sebum and comedones. Histological observations. Arch Dermatol. 1965 Oct;92(4):443–456. [PubMed] [Google Scholar]
  13. Troller J. A., Bozeman M. A. Isolation and characterization of a staphylococcal lipase. Appl Microbiol. 1970 Sep;20(3):480–484. doi: 10.1128/am.20.3.480-484.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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