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. 1971 May;21(5):893–898. doi: 10.1128/am.21.5.893-898.1971

Disc-Agar Diffusion Microbiological Assay Procedure for Determining Serum and Urine Levels of Sulfacytine and Other Sulfonamides

Carl L Heifetz 1, Josephine A Chodubski 1, Maureen O DeCarlo 1, Myron W Fisher 1
PMCID: PMC377304  PMID: 4930038

Abstract

A reasonably precise, reproducible, and sensitive microbiological procedure for directly assaying sulfacytine and other sulfonamides as antibacterially active drugs has been developed by appropriately modifying the standard disc-agar diffusion technique. Blood and urine levels as low as 3 μg/ml may be determined through the use of a strain of Escherichia coli and a chemically defined agar medium devoid of sulfonamide antagonists. Results indicate that this assay method should be a useful adjunct to the Bratton-Marshall colorimetric procedure, by permitting the direct measurement of antibacterially active drug in clinical specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTON A. H. The relation between the binding of sulfonamides to albumin and their antibacterial efficacy. J Pharmacol Exp Ther. 1960 Jul;129:282–290. [PubMed] [Google Scholar]
  2. BAUER A. W., SHERRIS J. C. THE DETERMINATION OF SULFONAMIDE SUSCEPTIBILITY OF BACTERIA. Chemotherapy. 1964;9(1):1–19. doi: 10.1159/000220337. [DOI] [PubMed] [Google Scholar]
  3. Batterman R. C., Tauber L. F., Bell M. E. Long-acting sulfonamides: in vivo correlation in man of protein binding, serum concentration and antimicrobial activity. Curr Ther Res Clin Exp. 1966 Mar;8(3):75–84. [PubMed] [Google Scholar]
  4. Doub L., Krolls U., Vandenbelt J. M., Fisher M. W. N-sulfanilyl-1-alkylcytosines. A new highly active class of "soluble," short-acting sulfanilamides. J Med Chem. 1970 Mar;13(2):242–246. doi: 10.1021/jm00296a018. [DOI] [PubMed] [Google Scholar]
  5. ERLANDSON A. L., Jr, FISHER M. W., GAGLIARDI L. A., PEARSON I. A., WAISBREN B. A. Characteristics of strains of Escherichia coli associated with severe infections in adults. J Infect Dis. 1961 Mar-Apr;108:189–194. doi: 10.1093/infdis/108.2.189. [DOI] [PubMed] [Google Scholar]
  6. KAPLAN K., CHEW W. H., WEINSTEIN L. STUDIES OF SULFAMETHOXYDIAZINE. II. ANTIBACTERIAL ACTIVITY. Clin Pharmacol Ther. 1965 May-Jun;6:316–320. doi: 10.1002/cpt196563316. [DOI] [PubMed] [Google Scholar]
  7. Krüger-Thiemer E., Bünger P., Dettli L., Spring P., Wempe E. Dosage regimen calculation of chemotherapeutic agents. 3. Sulfasymazine. Chemotherapy. 1965;10(6):325–338. doi: 10.1159/000220426. [DOI] [PubMed] [Google Scholar]
  8. Krüger-Thiemer E., Wempe E., Töpfer M. Die antibakterielle Wirkung des nicht eiweissgebundenen Anteils der Sulfanilamide im menschlichen Plasmawasser. Arzneimittelforschung. 1965 Nov;15(11):1309–1317. [PubMed] [Google Scholar]
  9. MOU T. W. Effect of urine pH on the antibacterial activity of antibiotics and chemotherapeutic agents. J Urol. 1962 Jun;87:978–987. doi: 10.1016/S0022-5347(17)65077-8. [DOI] [PubMed] [Google Scholar]
  10. Madsen S. T. Antibacterial effect of urine after single oral doses of sulfonamide. Chemotherapy. 1968;13(1):16–22. doi: 10.1159/000220526. [DOI] [PubMed] [Google Scholar]

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