Table 3.
Kinetic Properties of GST-Csk toward Selected Peptides
| Entry No. | Sequencea | kcat (s−1) | KM (μM) | kcat/KM (M−1 s−1) |
|---|---|---|---|---|
| 16 | KKKKEEIYFFF | ND | ND | 580 |
| 17 | FEEIDYVSPW | ND | ND | 390 |
| 18 | FEEPDYVEFI | ND | ND | 490 |
| 19 | EEEPEYIEPDDDE | ND | ND | 190 |
| 20 | FEEPEYIEPIDFE | ND | ND | 540 |
| 21 | EEEPEYAEIIVLP | ND | ND | 490 |
| 22 | DDEPEYIEFDAHN | ND | ND | 850 |
| 23 | DDEPIYAELADIT | 0.77 ± 0.10 | 120 ± 22 | 6300 |
| 24 | DDEPIYIEFDAHN | 0.72 ± 0.03 | 190 ± 19 | 3800 |
| 25 | FEEPIYIEPIDFE | 0.21 ± 0.01 | 150 ± 15 | 1400 |
| 26 | FEDSDYADIFIEE | ND | ND | 280 |
| 27 | FEDSIYADIFIEE | ND | ND | 320 |
| 28 | DDESEYINIPDGE | ND | ND | 130 |
| 29 | DDESIYINIPDGE | ND | ND | 180 |
a
All peptides contain an N-terminal acetyl group and a C-terminal amide.
ND, not determined.