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. 1971 Jun;21(6):1002–1006. doi: 10.1128/am.21.6.1002-1006.1971

Specific Assay of Aminoglycosidic- or Polymyxin-Type Antibiotics Present in Human Sera in Combination with Cephalosporins

S Ann Stroy 1, David A Preston 1
PMCID: PMC377332  PMID: 4327609

Abstract

Monitoring of serum concentrations of aminoglycosidic or polymyxin antibiotics when administered concurrently with cephalosporins or penicillins requires a special assay technique. Selective enzymatic degradation of the β-lactam antibiotic from the serum specimen allows subsequent assay of the antibiotic being monitored. This report gives details of a simple procedure for laboratory production of a crude enzyme capable of degrading cephalosporins or penicillins. An assay procedure for quantitating aminoglycosidic or polymyxin antibiotics after enzymatic degradation of a coexisting β-lactam antibiotic is described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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