Abstract
Monitoring of serum concentrations of aminoglycosidic or polymyxin antibiotics when administered concurrently with cephalosporins or penicillins requires a special assay technique. Selective enzymatic degradation of the β-lactam antibiotic from the serum specimen allows subsequent assay of the antibiotic being monitored. This report gives details of a simple procedure for laboratory production of a crude enzyme capable of degrading cephalosporins or penicillins. An assay procedure for quantitating aminoglycosidic or polymyxin antibiotics after enzymatic degradation of a coexisting β-lactam antibiotic is described.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ERIKSEN K. R., HANSEN D. Observations on the extracellular nature of staphylococcal penicillinase. Acta Pathol Microbiol Scand. 1954;35(2):169–174. doi: 10.1111/j.1699-0463.1954.tb00858.x. [DOI] [PubMed] [Google Scholar]
- Lane R. A., Cox F., McHenry M., Quinn E. L. Cephaloridine, laboratory and clinical evaluation. Antimicrob Agents Chemother (Bethesda) 1966;6:88–95. [PubMed] [Google Scholar]
- Newton G. G., Hamilton-Miller J. M. Cephaloridine: chemical and biochemical aspects. Postgrad Med J. 1967 Aug;43(Suppl):10–17. [PubMed] [Google Scholar]
- Sabath L. D., Abraham E. P. Cephalosporinase and penicillinase activity of Bacillus cereus. Antimicrob Agents Chemother (Bethesda) 1965;5:392–397. [PubMed] [Google Scholar]
- Stroy S. A. Modified microbiological assay for rapid estimation of antibiotic concentrations in human sera. Appl Microbiol. 1969 Jul;18(1):31–34. doi: 10.1128/am.18.1.31-34.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WICK W. E., HOLMES D. H., BONIECE W. S. Antipenicillinase serum: use in treatment of resistant staphylococcal infections in laboratory animals. Antibiot Chemother (Northfield) 1960 Feb;10:71–77. [PubMed] [Google Scholar]