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. Author manuscript; available in PMC: 2013 Dec 19.
Published in final edited form as: Neuron. 2013 Jun 19;78(6):1024–1035. doi: 10.1016/j.neuron.2013.04.036

Figure 8. Arc Is a Necessary but Not Sufficient Mediator of CF Synapse Elimination Downstream of P/Q-Type VDCC.

Figure 8

(A) Representative traces of CF-EPSCs from a PC with P/Q KD + Scr-Arc (upper) and that with P/Q KD + Arc KD (lower). A mixture of lentiviruses was injected at P2–3, and CF-EPSCs were recorded from 5 mice at P19–23 (P/Q KD + Scr-Arc) and 6 mice at P19–22 (P/Q KD + Arc KD).

(B) Frequency distribution histogram for the number of CFs innervating PCs with P/Q KD + Scr-Arc (gray columns, n = 38 PCs from 5 mice) and P/Q KD + Arc KD (filled columns, n = 47 PCs from 6 mice) showing no significant difference between the two groups of PCs (P = 0.9417, Mann-Whitney U test).

(C) Representative traces of CF-EPSCs from a PC with P/Q KD + lentivirus for mOrange expression (“mOrange”, upper) and that with P/Q KD + lentivirus for Arc overexpression (“Arc OE”, lower). A mixture of lentiviruses was injected at P2–3, and CF-EPSCs were recorded from 5 mice at P21–23 (P/Q KD + mOrange) and 7 mice at P20–23 (P/Q KD + Arc OE).

(D) Frequency distribution histogram for the number of CFs innervating PCs with P/Q KD + mOrange (gray columns, n = 35 PCs from 5 mice) and P/Q KD + Arc OE (filled columns, n = 49 PCs from 7 mice) showing no significant difference between the two groups of PCs (P = 0.4702, Mann-Whitney U test).

Scale bars, 10 ms and 1 nA. Holding potential, −10 mV.

See also Figure S7.