Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Jul;22(1):32–36. doi: 10.1128/am.22.1.32-36.1971

Microtiter Tests for Detecting Antibody in Bovine Serum to Parainfluenza 3 Virus, Infectious Bovine Rhinotracheitis Virus, and Bovine Virus Diarrhea Virus

C R Rossi 1, G K Kiesel 1
PMCID: PMC377372  PMID: 4329432

Abstract

Microneutralization tests for detection of antibody in bovine serum to three bovine viruses are described. The Madin-Darby bovine kidney cell line was used with parainfluenza 3 virus (PI 3), whereas serially cultivated bovine embryonic kidney cells were used for infectious bovine rhinotracheitis virus and bovine virus diarrhea virus. Comparison of micro-hemagglutination-inhibition (HI) with micro-serum-neutralization (SN) tests for PI 3 showed the SN test to be more sensitive, more specific, and therefore more useful than the HI test for detecting antibody. Although the effect of trypsin-periodate treatment of serum was to reduce the HI titer of numerous sera by a twofold dilution, sufficient evidence could not be found to indicate that nonspecific HI inhibitors to PI 3 are present in bovine sera.

Full text

PDF
32

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABINANTI F. R., HOERLEIN A. B., WATSON R. L., HUEBNER R. J. Serologic studies on myxovirus para-influenza 3 in cattle and the prevalence of antibodies in bovines. J Immunol. 1961 May;86:505–511. [PubMed] [Google Scholar]
  2. DAWSON P. S. THE NATURE OF SUBSTANCES PRESENT IN NORMAL BOVINE SERA INHIBITING THE ACTIVITY OF PARAINFLUENZA 3 VIRUS. J Comp Pathol. 1963 Oct;73:428–436. doi: 10.1016/s0368-1742(63)80045-4. [DOI] [PubMed] [Google Scholar]
  3. HAMPARIAN V. V., WASHKO F. V., KETLER A., HILLEMAN M. R. Laboratory and field investigations of bovine Myxovirus parainfluenza 3 virus and vaccine. III. Evaluation of an SF-4 (shipping fever) virus vaccine in cattle. J Immunol. 1961 Aug;87:139–146. [PubMed] [Google Scholar]
  4. HOERLEIN A. B., MANSFIELD M. E., ABINANTI F. R., HUEBNER R. J. Studies of shipping fever of cattle. I. Para-influenza 3 virus antibodies in feeder calves. J Am Vet Med Assoc. 1959 Aug 1;135(3):153–160. [PubMed] [Google Scholar]
  5. Hierholzer J. C., Suggs M. T. Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions. Appl Microbiol. 1969 Nov;18(5):816–823. doi: 10.1128/am.18.5.816-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KETLER A., HAMPARIAN V. V., HILLEMAN M. R. Laboratory and field investigations of bovine Myxovirus parainfluenza 3 virus and vaccine. I. Properties of the SF-4 (shipping fever) strain of virus. J Immunol. 1961 Aug;87:126–133. [PubMed] [Google Scholar]
  7. ROSENBAUM M. J., PHILLIPS I. A., SULLIVAN E. J., EDWARDS E. A., MILLER L. F. A simplified method for virus-tissue culture procedures in microtitration plates. Proc Soc Exp Biol Med. 1963 May;113:224–229. doi: 10.3181/00379727-113-28325. [DOI] [PubMed] [Google Scholar]
  8. Rosenbaum M. J., Edwards E. A., Sullivan E. J. Micromethods for respiratory virus sero-epidemiology. Health Lab Sci. 1970 Jan;7(1):42–52. [PubMed] [Google Scholar]
  9. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  10. Sullivan E. J., Rosenbaum M. J. Methods for preparing tissue culture in disposable microplates and their use in virology. Am J Epidemiol. 1967 May;85(3):424–437. [PubMed] [Google Scholar]
  11. TAKATSY G. The use of spiral loops in serological and virological micro-methods. Acta Microbiol Acad Sci Hung. 1955;3(1-2):191–202. [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES