Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1971 Jul;22(1):145–146. doi: 10.1128/am.22.1.145-146.1971

Growth of Human Lymphoid Cells (Raji Strain) in a Five-Liter Fermentor

Frederick Klein 1, Bill G Mahlandt 1, Ralph E Lincoln 1
PMCID: PMC377397  PMID: 5111304

Abstract

Procedures used to produce an established line of mammalian cells (L cell) in a New Brunswick fermentor were adapted to propagate human lymphoid cells in suspended culture. Control of pH within defined limits was more effective for regulation of cell metabolism than control of oxidation-reduction potential. An unusually high rate of agitation was required.

Full text

PDF
145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Higuchi K. An improved chemically defined culture medium for strain L mouse cells based on growth responses to graded levels of nutrients including iron and zinc ions. J Cell Physiol. 1970 Feb;75(1):65–72. doi: 10.1002/jcp.1040750108. [DOI] [PubMed] [Google Scholar]
  2. PULVERTAFT J. V. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965 May;18:261–273. doi: 10.1136/jcp.18.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES