Abstract
New methods of sterilization employing a chemical with moderate heat and ultrasonic energy have been devised. Inactivation of high-density bacterial spore suspensions is achieved by treatment with low concentration aqueous acid glutaraldehyde solutions at temperatures above or about 54 C. Low (20 kHz) or high (250 kHz) frequency ultrasonic energy is synergistic with glutaraldehyde. Rapid inactivation may also be achieved by using ultrasonic energy and aqueous alkalinized glutaraldehyde solutions at low (25 C) or moderate (55 C) temperatures. If compared to present room temperature techniques, “surface sterilization” time for contaminated objects can be reduced from hours to minutes.
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Selected References
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- Boucher R. M., Pisano M. A., Tortora G., Sawicki E. Synergistic effects in sonochemical sterilization. Appl Microbiol. 1967 Nov;15(6):1257–1261. doi: 10.1128/am.15.6.1257-1261.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubbo S. D., Gardner J. F., Webb R. L. Biocidal activities of glutaraldehyde and related compounds. J Appl Bacteriol. 1967 Apr;30(1):78–87. doi: 10.1111/j.1365-2672.1967.tb00277.x. [DOI] [PubMed] [Google Scholar]
- Sierra G., Bowman A. Mechanism and factors influencing the veronal inhibition of bacterial spore germination. Appl Microbiol. 1969 Mar;17(3):372–378. doi: 10.1128/am.17.3.372-378.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sierra G. Germination of bacterial endospores with subtilopeptidases. Can J Microbiol. 1967 May;13(5):489–501. doi: 10.1139/m67-064. [DOI] [PubMed] [Google Scholar]