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. 1971 Aug;22(2):233–241. doi: 10.1128/am.22.2.233-241.1971

Beta Hemolysin: a Persistent Impurity in Preparations of Staphylococcal Nuclease and Enterotoxin

William Chesbro 1, Vera Kucic 1
PMCID: PMC377420  PMID: 4999525

Abstract

Purified staphylococcal nuclease and enterotoxin B from several sources contained beta hemolysin whose physicochemical resemblances to the other two proteins make its elimination difficult.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER M., HEPPEL L. A., HURWITZ J. The purification and properties of micrococcal nuclease. J Biol Chem. 1961 Nov;236:3014–3019. [PubMed] [Google Scholar]
  2. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BAIRD-PARKER A. C., JOSEPH R. L. FRACTIONATION OF STAPHYLOCOCCAL ENTEROTOXIN B. Nature. 1964 May 9;202:570–571. doi: 10.1038/202570a0. [DOI] [PubMed] [Google Scholar]
  4. BERGDOLL M. S., SUGIYAMA H., DACK G. M. Staphylococcal enterotoxin. I. Purification. Arch Biochem Biophys. 1959 Nov;85:62–69. doi: 10.1016/0003-9861(59)90447-3. [DOI] [PubMed] [Google Scholar]
  5. BERGDOLL M. S., SURGALLA M. J., DACK G. M. Staphylococcal enterotoxin. Identification of a specific precipitating antibody with enterotoxin-neutralizing property. J Immunol. 1959 Sep;83:334–338. [PubMed] [Google Scholar]
  6. BURNS J., HOLTMAN D. F. Biochemical properties of virulent and avirulent staphylococci. Ann N Y Acad Sci. 1960 Nov 21;88:1115–1124. doi: 10.1111/j.1749-6632.1960.tb20101.x. [DOI] [PubMed] [Google Scholar]
  7. CASMAN E. P. Serologic studies of staphylococcal enterotoxin. Public Health Rep. 1958 Jul;73(7):599–609. [PMC free article] [PubMed] [Google Scholar]
  8. CHESBRO W. R., HEYDRICK F. P., MARTINEAU R., PERKINS G. N. PURIFICATION OF STAPHYLOCOCCAL BETA-HEMOLYSIN AND ITS ACTION ON STAPHYLOCOCCAL AND STREPTOCOCCAL CELL WALLS. J Bacteriol. 1965 Feb;89:378–389. doi: 10.1128/jb.89.2.378-389.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. CLARK W. G., VANDERHOOFT G. F., BORISON H. L. Emetic effect of purified staphylococcal enterotoxin in cats. Proc Soc Exp Biol Med. 1962 Oct;111:205–207. doi: 10.3181/00379727-111-27745. [DOI] [PubMed] [Google Scholar]
  10. COOPER L. Z., MADOFF M. A., WEINSTEIN L. HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN. I. KINETICS OF THE LYTIC REACTION. J Bacteriol. 1964 Jan;87:127–135. doi: 10.1128/jb.87.1.127-135.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Casman E. P., Bennett R. W., Dorsey A. E., Issa J. A. Identification of a fourth staphylococcal enterotoxin, enterotoxin D. J Bacteriol. 1967 Dec;94(6):1875–1882. doi: 10.1128/jb.94.6.1875-1882.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Chesbro W. R., Auborn K. Enzymatic detection of the growth of Staphylococcus aureus in foods. Appl Microbiol. 1967 Sep;15(5):1150–1159. doi: 10.1128/am.15.5.1150-1159.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Cuatrecasas P., Fuchs S., Anfinsen C. B. Cross-linking of aminotyrosyl residues in the active site of staphylococcal nuclease. J Biol Chem. 1969 Jan 25;244(2):406–412. [PubMed] [Google Scholar]
  14. Cuatrecasas P., Wilchek M., Anfinsen C. B. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636–643. doi: 10.1073/pnas.61.2.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Doery H. M., Magnusson B. J., Gulasekharam J., Pearson J. E. The properties of phospholipase enzymes in staphylococcal toxins. J Gen Microbiol. 1965 Aug;40(2):283–296. doi: 10.1099/00221287-40-2-283. [DOI] [PubMed] [Google Scholar]
  16. FREA J. I., MCCOY E., STRONG F. M. PURIFICATION OF TYPE B STAPHYLOCOCCAL ENTEROTOXIN. J Bacteriol. 1963 Dec;86:1308–1313. doi: 10.1128/jb.86.6.1308-1313.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gow J. A., Robinson J. Properties of purified staphylococcal beta-hemolysin. J Bacteriol. 1969 Mar;97(3):1026–1032. doi: 10.1128/jb.97.3.1026-1032.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. HALL H. E., ANGELOTTI R., LEWIS K. H. DETECTION OF THE STAPHYLOCOCCAL ENTEROTOXINS IN FOOD. Health Lab Sci. 1965 Jul;2:179–191. [PubMed] [Google Scholar]
  19. Hacha R., Fredericq E. Purification and some enzymic properties of Staphylococcus aureus deoxyribonuclease. Biochim Biophys Acta. 1966 Sep;123(3):493–502. doi: 10.1016/0005-2787(66)90217-6. [DOI] [PubMed] [Google Scholar]
  20. Haque R. U., Baldwin J. N. Purification and properties of staphylococcal beta hemolysin. II. Purification of beta hemolysin. J Bacteriol. 1969 Nov;100(2):751–759. doi: 10.1128/jb.100.2.751-759.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Heins J. N., Suriano J. R., Taniuchi H., Anfinsen C. B. Characterization of a nuclease produced by Staphylococcus aureus. J Biol Chem. 1967 Mar 10;242(5):1016–1020. [PubMed] [Google Scholar]
  22. Johnson H. M., Hall H. E., Simon M. Enterotoxin B: serological assay in cultures by passive hemagglutination. Appl Microbiol. 1967 Jul;15(4):815–818. doi: 10.1128/am.15.4.815-818.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  24. MUKAI J., OHSAKA A., MCEVOY C., LASKOWSKI M., Sr FURTHER PURIFICATION OF MICROCOCCAL NUCLEASE FROM THE STRAIN SA-B. Biochem Biophys Res Commun. 1965 Jan 4;18:136–140. doi: 10.1016/0006-291x(65)90896-x. [DOI] [PubMed] [Google Scholar]
  25. Martin W. J., Marcus S. Relation of pyrogenic and emetic properties of enterobacteriaceal endotoxin and of staphylococcal enterotoxin. J Bacteriol. 1964 May;87(5):1019–1026. doi: 10.1128/jb.87.5.1019-1026.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Morse S. A., Mah R. A., Dobrogosz W. J. Regulation of staphylococcal enterotoxin B. J Bacteriol. 1969 Apr;98(1):4–9. doi: 10.1128/jb.98.1.4-9.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. OHSAKA A., MUKAI J. I., LASKOWSKI M., Sr THE USE OF PURIFIED MICROCOCCAL NUCLEASE IN IDENTIFYING THE NUCLEOTIDE TERMINUS BEARING A FREE 5'-MONOPHOSPHATE. J Biol Chem. 1964 Oct;239:3498–3504. [PubMed] [Google Scholar]
  28. PREER J. R., Jr A quantitative study of a technique of double diffusion in agar. J Immunol. 1956 Jul;77(1):52–60. [PubMed] [Google Scholar]
  29. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
  30. Silverman S. J., Knott A. R., Howard M. Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods. Appl Microbiol. 1968 Jul;16(7):1019–1023. doi: 10.21236/ad0838753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Spero L., Stefanye D., Brecher P. I., Jacoby H. M., Dalidowicz J. E., Schantz E. J. Amino acid composition and terminal amino acids of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1024–1030. doi: 10.1021/bi00882a007. [DOI] [PubMed] [Google Scholar]
  32. Sulkowski E., Laskowski M., Sr Phosphatase-free crystalline micrococcal nuclease. J Biol Chem. 1966 Oct 10;241(19):4386–4388. [PubMed] [Google Scholar]
  33. Taniuchi H., Anfinsen C. B., Sodja A. The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. 3. Complete amino acid sequence. J Biol Chem. 1967 Oct 25;242(20):4752–4758. [PubMed] [Google Scholar]
  34. Wadström T. Studies on extracellular proteins from Staphylococcus aureus. II. Separation of deoxyribonucleases by isoelectric focusing. Purification and properties of the enzymes. Biochim Biophys Acta. 1967 Dec 12;147(3):441–452. [PubMed] [Google Scholar]
  35. Wagman J., Edwards R. C., Schantz E. J. Molecular size, homogeneity, and hydrodynamic properties of purified staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1017–1023. doi: 10.1021/bi00882a006. [DOI] [PubMed] [Google Scholar]
  36. Weirether F. J., Lewis E. E., Rosenwald A. J., Lincoln R. E. Rapid quantitative serological assay of staphylococcal enterotoxin B. Appl Microbiol. 1966 Mar;14(2):284–291. doi: 10.1128/am.14.2.284-291.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

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