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. 1969 Jan;17(1):31–33. doi: 10.1128/am.17.1.31-33.1969

Acrylamide Gel Electrophoresis of Group A Streptococcal Cell Walls

Sandra A Larsen 1, C Douglas Webb 1, Max D Moody 1
PMCID: PMC377607  PMID: 4888625

Abstract

Patterns obtained by acrylamide gel electrophoresis of group A streptococcal cell walls were characteristic for M types 1, 2, 3, 4, and 5, respectively. Best results were obtained with cell walls which had been solubilized with a phenol-acetic acid-water mixture. The method will make it possible to compare group A streptococci of epidemiological and clinical significance and to separate components for more critical analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLEIWEIS A. S., KARAKAWA W. W., KRAUSE R. M. IMPROVED TECHNIQUE FOR THE PREPARATION OF STREPTOCOCCAL CELL WALLS. J Bacteriol. 1964 Oct;88:1198–1200. doi: 10.1128/jb.88.4.1198-1200.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. LANCEFIELD R. C. Current knowledge of type-specific M antigens of group A streptococci. J Immunol. 1962 Sep;89:307–313. [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Liu T. Y., Gotschlich E. C. Muramic acid phosphate as a component of the mucopeptide of Gram-positive bacteria. J Biol Chem. 1967 Feb 10;242(3):471–476. [PubMed] [Google Scholar]
  6. MOODY M. D., PADULA J., LIZANA D., HALL C. T. EPIDEMIOLOGIC CHARACTERIZATION OF GROUP A STREPTOCOCCI BY T-AGGLUTINATION AND M-PRECIPITATION TESTS IN THE PUBLIC HEALTH LABORATORY. Health Lab Sci. 1965 Jul;2:149–162. [PubMed] [Google Scholar]
  7. Rottem S., Razin S. Electrophoretic patterns of membrane proteins of Mycoplasma. J Bacteriol. 1967 Aug;94(2):359–364. doi: 10.1128/jb.94.2.359-364.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. WILSON A. T., WILEY G. G. THE CELLULAR ANTIGENS OF GROUP A STREPTOCOCCI; IMMUNOELECTROPHORETIC STUDIES OF THE C, M, T, PGP, E4, F, AND E ANTIGENS OF SEROTYPE 17 STREPTOCOCCI. J Exp Med. 1963 Oct 1;118:527–556. doi: 10.1084/jem.118.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]

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