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. 1969 Jan;17(1):34–40. doi: 10.1128/am.17.1.34-40.1969

Micro Radiometric Method for Study of Acid-insoluble Materials in Monolayer Cell Cultures

Donald W Ziegler 1, Harriet D Hutchinson 1, Erskine L Palmer 1
PMCID: PMC377608  PMID: 5774761

Abstract

A simple radiometric procedure for study of acid-insoluble products synthesized in monolayer cell cultures is described. Cell cultures were produced directly on the bottom surface of scintillation vials or on glass cover slips (8 X 30 mm). The cells were labeled and extracted; the radioactivity was determined while the cells remained affixed to the glass surface upon which they were grown. This procedure enabled rapid investigations of certain biosynthetic processes to be carried out by using many individual cell cultures. The method was applied to an investigation of 3H-thymidine incorporation induced by vaccinia virus in a 5-bromodeoxyuridine-resistant cell line. 14C-labeling was evaluated as an alternate procedure for cell quantitation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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