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. 1969 Jan;17(1):41–47. doi: 10.1128/am.17.1.41-47.1969

Vaccinia Neutralization Test Using a Radioisotope Assay for Virus-induced Enzyme Activity

Donald W Ziegler 1, Harriet D Hutchinson 1
PMCID: PMC377609  PMID: 5774762

Abstract

Vaccinia virus induction of a metabolic activity in host cell cultures forms the basis of a new assay for neutralizing antibodies. A direct relationship between the amount of vaccinia virus infecting cell cultures and the induced incorporation of tritium-labeled thymidine into the acid-insoluble fraction of the cells provided an indicator system. A liquid scintillation spectrometer was used to determine radioactivity associated with cell materials, and it provided a method for partial automation of an immunological procedure. Reproducibility of the method was satisfactory, and agreement with conventional vaccinia serum neutralization tests was demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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