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. 1969 Jan;17(1):102–105. doi: 10.1128/am.17.1.102-105.1969

Simple Isolation of Antimycin A1 and Some of Its Toxicological Properties

P B Hamilton a,1, F I Carroll a, J H Rutledge a, J E Mason a, B S H Harris a, C S Fenske a, M E Wall a
PMCID: PMC377621  PMID: 5774751

Abstract

An unidentified actinomycete, RTI 246, was found to produce antimycin A1 in high yield on a high protein cereal medium. The antibiotic compound was extracted from the cells and isolated in pure form by crystallization. It was identified by ultraviolet, infrared, nuclear magnetic resonance, and mass spectroscopy and by alkaline hydrolysis to antimycic acid and a neutral lactone. The intravenous LD50 was 1.0 mg/kg in white mice, whereas the intraperitoneal LD50 was 1.50 ± 0.19 mg/kg. Animals receiving an intraperitoneal injection displayed an incoordination of the hind limbs and impaired reflexes before showing signs of respiratory distress. These findings indicated that antimycin A1 possesses a neurotoxic property separate from its well-documented property as a respiratory poison.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AHMAD K., SCHNEIDER H. G., STRONG F. M. Studies on the biological action of antimycin A. Arch Biochem. 1950 Sep;28(2):281–294. [PubMed] [Google Scholar]
  2. DERSE P. H., STRONG F. M. TOXICITY OF ANTIMYCIN TO FISH. Nature. 1963 Nov 9;200:600–601. doi: 10.1038/200600b0. [DOI] [PubMed] [Google Scholar]
  3. ERNSTER L., LEE C. P. BIOLOGICAL OXIDOREDUCTIONS. Annu Rev Biochem. 1964;33:729–790. doi: 10.1146/annurev.bi.33.070164.003501. [DOI] [PubMed] [Google Scholar]
  4. Giacobini E. The effect of metabolic and ion transport inhibitors on the impulse activity and oxygen uptake of an isolated crustacean neurone. Acta Physiol Scand. 1966 Jan-Feb;66(1):34–48. doi: 10.1111/j.1748-1716.1966.tb03165.x. [DOI] [PubMed] [Google Scholar]
  5. KIDO G. S., SPYHALSKI E. Antimycin A, an antibiotic with insecticidal and miticidal properties. Science. 1950 Aug 11;112(2902):172–173. doi: 10.1126/science.112.2902.172. [DOI] [PubMed] [Google Scholar]
  6. NAKAYAMA K., OKAMOTO F., HARADA Y. Antimycin A: isolation from a new Streptomyces and activity against rice plant blast fungi. J Antibiot (Tokyo) 1956 Mar;9(2):63–66. [PubMed] [Google Scholar]
  7. SAKAGAMI Y., TAKEUCHI S., YONEHARA H., SAKAI H., TAKASHIMA M. Antifungal substances, No. 720-A and No. 720-B, and probable identity of No. 720-A with antimycin A, virosin, and antipiricullin. J Antibiot (Tokyo) 1956 Jan;9(1):1–5. [PubMed] [Google Scholar]
  8. TAPPEL A. L. Inhibition of electron transport by antimycin A, alkyl hydroxy naphthoquinones and metal coordination compounds. Biochem Pharmacol. 1960 Jul;3:289–296. doi: 10.1016/0006-2952(60)90094-0. [DOI] [PubMed] [Google Scholar]
  9. Vezina C. Antimycin A, a teleocidal antibiotic. Antimicrob Agents Chemother (Bethesda) 1966;6:757–766. doi: 10.1128/AAC.6.6.757. [DOI] [PubMed] [Google Scholar]
  10. WATANABE K., TANAKA T., FUKUHARA K., MIYAIRI N., YONEHARA H., UMEZAWA H. Blastmycin, a new antibiotic from Streptomyces sp. J Antibiot (Tokyo) 1957 Mar;10(2):39–45. [PubMed] [Google Scholar]

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