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Representative multicolor fluorescence microscopy images of human HeLa cells after they were treated with Tetra-SR-bZnDPA (10 µM) for 15 minutes followed by rapid sequential addition of Calcein AM (1 µg/mL) and bacterial E. coli K12 cells. Brightfield image (A); green fluorescence indicating viable human cells (B); deep-red fluorescence indicating bacteria selectively stained and agglutinated by Tetra-SR-bZnDPA (C); composite image (D). Scale bar = 30 μm.
