5′:agcacagacacacgggagacaatgggtaagaaagagagggggaaggagagcacatagcgaacccatctatcctttggatattgtgga aaacaaacatttagaaagtgaagtcaatcccatcctgtattcaccatgatcgccctatacagaaatcacacaaagtctcttattgaggtaattatg ggccactgccttagcgttccacagaaaatcttgtttggcctacccaggatttataggggaagtgaaatcattaaataactagttaacctctgttt ggcaaaaagagtaagtaattactgaaaaggggagaaagaaaaaaa>>4aaaaaaaaaccctgcctttcacaaacagcacagatagcatc ctgaagggatttacacaatacaccaacg85tttgttctgtacccagagttgttaaaataattgtgatgacaggtgtctaggtaccactttcttaat ggttttcaatatccattgatttattattgtggctccccccacttacatctctagctctaagaaggggctaagaaaagagattttgcaccaaaggct gattctttattcctgggctgaaaagaactaataaaaataatcactcg84gcttttaagcagaaagcttccatttacagtatttcacccttacccccc ctccaagccccttgttatctaacataatggctagaatacatttccg83tctctccccttaataacttc>>3atg4<<cattaacatcctcttcacac atgcgcgtgcgtgcgcg78cg77cg76cg75cg74cg73cg72cacacacacacacacacacacagaggcaaacgcgcgcgcacccagcaaa aggcggaggtgggggggaaagaggcagcagaaatctcagctgtacttctagc3<<ccttttaaaaagtttgctctgtaaattggaatgag gtcagatttggagcttctcattgcacgcggagattattattgcatcgggttccaagccaatgggaaggacgggggaggggcttggcacgag gaagcgttagttacagcggctgattggctgtctgagaaaggataaagaagcgcgaggcggaattggggtctgctctaagctgcagccaga gaaacagagtgaggggaagagggccgtctccctcccggtttccagttcttgcacgctgtttcttagagagtctgcagtgggggaactctgcc ggtaaccagctccccttcttgcaggagggagggagaaacatacatttattcatgccggtctgttgcatgcaagcttcttggcttcctaccttgca acaaaataattgcaccaactcctcagcgccgattccgcccacagagagtcccggagccagagtcgctttggctttgcactgcaggaaaggg acttaggcgctagagacgatgtcgctttcctgagctaccgcgagctctcgtgaactgcaatcgactgcttcagggaaaggggtgggggaaa gacttgccccggaggcggcgagaaacttgcgtttggaagatactccggctaccaacgtttggagaaactcctcgccgcggccgactccag cctcggtgcccgcagggagcacttcagcggtgagggaggacggagggcctcggggactctaggttggcggcgggaggcggccgcccc tggcccgcgcgccgctcaggggaccctggtctccgccccggggagcccgcagggcccggcgaccgcgccgcgagcgtgtgagcgcgc gtgggcgcccggcaag:3′

Bold indicates CpG sites.

Bold and underline indicate CpG sites of amplicons 3 and 4 exhibiting differential methylation.

>>3: Start of amplicon 3; >>4: Start of amplicon 4; 3<<: End of amplicon 3; 4<<: End of amplicon 4.