Abstract
An Amber MPH-Cerelose medium was devised on which the unidentified basidiomycete B 191039 produced high titers of alcohol oxidase in shake cultures. Another, fully synthetic medium was developed on which the production was nearly as good, but very slow. No cofactors were required to produce this flavine adenine dinucleotide-containing enzyme. The B 191039 organism underwent frequent mutations. The mutant forms were morphologically distinct from the parent strain and elaborated little or no alcohol oxidase. Several other basidiomycetes were shown to produce alcohol oxidase in submerged culture.
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Selected References
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- Espenshade M. A., Griffith E. W. Tumor-inhibiting basidiomycetes. Isolation and cultivation in the laboratory. Mycologia. 1966 Jul-Aug;58(4):511–517. [PubMed] [Google Scholar]
- Gregory F. J., Healy E. M., Agersborg H. P., Jr, Warren G. H. Studies on antitumor substances produced by Basidiomycetes. Mycologia. 1966 Jan-Feb;58(1):80–90. [PubMed] [Google Scholar]
- Janssen F. W., Kerwin R. M., Ruelius H. W. Alcohol oxidase, a novel enzyme from a basidiomycete. Biochem Biophys Res Commun. 1965 Sep 8;20(5):630–634. doi: 10.1016/0006-291x(65)90446-8. [DOI] [PubMed] [Google Scholar]
- Janssen F. W., Ruelius H. W. Alcohol oxidase, a flavoprotein from several Basidiomycetes species. Crystallization by fractional precipitation with polyethylene glycol. Biochim Biophys Acta. 1968 Feb 5;151(2):330–342. doi: 10.1016/0005-2744(68)90100-9. [DOI] [PubMed] [Google Scholar]
- Ruelius H. W., Janssen F. W., Kerwin R. M., Goodwin C. W., Schillings R. T. Poricin, an acidic protein with antitumor activity from a basidiomycete. I. Production, isolation, and purification. Arch Biochem Biophys. 1968 Apr;125(1):126–135. doi: 10.1016/0003-9861(68)90646-2. [DOI] [PubMed] [Google Scholar]
- Ruelius H. W., Kerwin R. M., Janssen F. W. Carbohydrate oxidase, a novel enzyme from Polyporus obtusus. I. Isolation and purification. Biochim Biophys Acta. 1968 Nov 19;167(3):493–500. doi: 10.1016/0005-2744(68)90039-9. [DOI] [PubMed] [Google Scholar]