Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1969 Mar;17(3):384–393. doi: 10.1128/am.17.3.384-393.1969

Rapid Identification of Viruses by Indirect Immunofluorescence: Standardization and Use of Antiserum Pool to Nine Respiratory Viruses

T D Stevens 1, H M S Watkins 1
PMCID: PMC377698  PMID: 4305395

Abstract

The indirect fluorescent antibody test employing treated and standardized antisera and conjugated antiglobulin has been used successfully, in conjunction with a technique for growing and staining virus cell systems in situ on microscope slides, in the identification of nine respiratory viruses. By using pooled antisera in a single test, the presence or absence of these viruses was determined in 18 to 45 hr after inoculation of slide microtissue culture.

Full text

PDF
384

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROWN G. C., MAASSAB H. F., VERONELLI J. A., FRANCIS T. J., Jr RUBELLA ANTIBODIES IN HUMAN SERUM: DETECTION BY THE INDIRECT FLUORESCENT ANTIBODY TECHNIC. Science. 1964 Aug 28;145(3635):943–945. doi: 10.1126/science.145.3635.943. [DOI] [PubMed] [Google Scholar]
  2. COATES H. V., CHANOCK R. M. Experimental infection with respiratory syncytial virus in several species of animals. Am J Hyg. 1962 Nov;76:302–312. doi: 10.1093/oxfordjournals.aje.a120285. [DOI] [PubMed] [Google Scholar]
  3. COOK M. K., ANDREWS B. E., FOX H. H., TURNER H. C., JAMES W. D., CHANOCK R. M. Antigenic relationships among the newer myxoviruses (parainfluenza). Am J Hyg. 1959 May;69(3):250–264. doi: 10.1093/oxfordjournals.aje.a119998. [DOI] [PubMed] [Google Scholar]
  4. HAMPARIAN V. V., MULLER F., HUMMELER K. Elimination of nonspecific components from viral antigens by fluorocarbon. J Immunol. 1958 Jun;80(6):468–475. [PubMed] [Google Scholar]
  5. HERS J. F. FLUORESCENT ANTIBODY TECHNIQUE IN RESPIRATORY VIRAL DISEASES. Am Rev Respir Dis. 1963 Sep;88:SUPPL–338. doi: 10.1164/arrd.1963.88.3P2.316. [DOI] [PubMed] [Google Scholar]
  6. HUMMELER K. Mumps complement fixing antibodies in guinea pigs. J Immunol. 1957 Oct;79(4):337–341. [PubMed] [Google Scholar]
  7. Hamre D., Connelly A. P., Jr, Procknow J. J. Virologic studies of acute respiratory disease in young adults. IV. Virus isolations during four years of surveillance. Am J Epidemiol. 1966 Mar;83(2):238–249. doi: 10.1093/oxfordjournals.aje.a120579. [DOI] [PubMed] [Google Scholar]
  8. Kissling R. E., Robinson R. Q., Murphy F. A., Whitfield S. G. Agent of disease contracted from green monkeys. Science. 1968 May 24;160(3830):888–890. doi: 10.1126/science.160.3830.888. [DOI] [PubMed] [Google Scholar]
  9. LEVY H. B., SOBER H. A. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. doi: 10.3181/00379727-103-25476. [DOI] [PubMed] [Google Scholar]
  10. LIEF F. S., FABIYI A., HENLE W. Antigenic analyses of influenza viruses by complement fixation. I. The production of antibodies to the soluble antigen in guinea pigs. J Immunol. 1958 Jan;80(1):53–65. [PubMed] [Google Scholar]
  11. LIM K. A., BENYESH-MELNICK M. Typing of viruses by combinations of antiserum pools. Application to typing of enteroviruses (Coxsackie and ECHO). J Immunol. 1960 Mar;84:309–317. [PubMed] [Google Scholar]
  12. PITAL A., JANOWITZ S. L. ENHANCEMENT OF STAINING INTENSITY IN THE FLUORESCENT-ANTIBODY REACTION. J Bacteriol. 1963 Oct;86:888–889. doi: 10.1128/jb.86.4.888-889.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RIGGS J. L., LOH P. C., EVELAND W. C. A simple fractionation method for preparation of fluorescein-labeled gamma globulin. Proc Soc Exp Biol Med. 1960 Dec;105:655–658. doi: 10.3181/00379727-105-26207. [DOI] [PubMed] [Google Scholar]
  14. SHAW E. D., NEWTON A., POWELL A. W., FRIDAY C. J. Fluorescent antigen-antibody reactions in Coxsackie and ECHO enteroviruses. Virology. 1961 Oct;15:208–210. doi: 10.1016/0042-6822(61)90239-2. [DOI] [PubMed] [Google Scholar]
  15. Sommerville R. G. Rapid method for the preparation of replicate microslide tissue culture to facilitate immunofluorescent identification of unknown virus isolates. J Clin Pathol. 1967 Mar;20(2):212–214. doi: 10.1136/jcp.20.2.212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sommerville R. G. The production of fluorescent antibody reagents for virus diagnosis in the albino mouse. I. Hyperimmune anti-species serum. Arch Gesamte Virusforsch. 1967;20(4):445–451. doi: 10.1007/BF01275225. [DOI] [PubMed] [Google Scholar]
  17. Sommerville R. G. The production of fluorescent antibody reagents for virus diagnosis in the albino mouse. II. Coupling of mouse immune globulin with fluorescein isothiocyanate (FITC). Arch Gesamte Virusforsch. 1967;20(4):452–458. doi: 10.1007/BF01275226. [DOI] [PubMed] [Google Scholar]
  18. WELLER T. H., COONS A. H. Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):789–794. doi: 10.3181/00379727-86-21235. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES