Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1969 Mar;17(3):422–426. doi: 10.1128/am.17.3.422-426.1969

Use of Erythrocytes Sensitized with Purified Pneumococcal Polysaccharides for the Assay of Antibody and Antibody-producing Cells

Phillip J Baker 1,2, Philip W Stashak 1,2, Benjamin Prescott 1,2
PMCID: PMC377705  PMID: 4388600

Abstract

A method was described for the sensitization of erythrocytes with purified type-specific pneumococcal polysaccharide antigens using chromium chloride as a coupling agent. Erythrocytes so sensitized can be used in routine passive hemagglutination and hemolysis tests as well as in the technique of localized hemolysis-in-gel for the detection of specific antibody and specific antibody-producing cells, respectively.

Full text

PDF
422

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASKONAS B. A., FARTHING C. P., HUMPHREY J. H. The significance of multiple antibody components in serum of immunized rabbits. Immunology. 1960 Oct;3:336–351. [PMC free article] [PubMed] [Google Scholar]
  2. BROOKE M. S. BREAKING OF IMMUNOLOGICAL PARALYSIS BY INJECTION OF A SPECIFIC DEPOLYMERASE. Nature. 1964 Dec 26;204:1319–1320. doi: 10.1038/2041319a0. [DOI] [PubMed] [Google Scholar]
  3. Brooke M. S. A hemagglutination test and a specific depolymerase for studying immunologic paralysis in mice. J Immunol. 1966 Feb;96(2):358–363. [PubMed] [Google Scholar]
  4. Brooke M. S. Studies on the induction, specificity, prevention and breaking of immunologic paralysis and immunity to pneumococcal polysaccharide. J Immunol. 1966 Feb;96(2):364–372. [PubMed] [Google Scholar]
  5. DAVIES D. A., CRUMPTON M. J., MACPHERSON I. A., HUTCHISON A. M. The adsorption of bacterial polysaccharides by erythrocytes. Immunology. 1958 Apr;1(2):157–171. [PMC free article] [PubMed] [Google Scholar]
  6. FELTON L. D., KAUFFMANN G., PRESCOTT B., OTTINGER B. Studies on the mechanism of the immunological paralysis induced in mice by pneumococcal polysaccharides. J Immunol. 1955 Jan;74(1):17–26. [PubMed] [Google Scholar]
  7. Gold E. R., Fudenberg H. H. Chromic chloride: a coupling reagent for passive hemagglutination reactions. J Immunol. 1967 Nov;99(5):859–866. [PubMed] [Google Scholar]
  8. LANDY M., TRAPANI R. J., CLARK W. R. Studies on the O antigen of Salmonella typhosa. III. Activity of the isolated antigen in the hemagglutination procedure. Am J Hyg. 1955 Jul;62(1):54–65. doi: 10.1093/oxfordjournals.aje.a119766. [DOI] [PubMed] [Google Scholar]
  9. Landy M., Sanderson R. P., Jackson A. L. Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis. J Exp Med. 1965 Sep 1;122(3):483–504. doi: 10.1084/jem.122.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NETER E. Bacterial hemagglutination and hemolysis. Bacteriol Rev. 1956 Sep;20(3):166–188. doi: 10.1128/br.20.3.166-188.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TAKATSY G. The use of spiral loops in serological and virological micro-methods. Acta Microbiol Acad Sci Hung. 1955;3(1-2):191–202. [PubMed] [Google Scholar]
  12. WOLFF S. M., WARD S. B., LANDY M. SEROLOGIC PROPERTIES OF BENTONITE PARTICLES COATED WITH MICROBIAL POLYSACCHARIDES. Proc Soc Exp Biol Med. 1963 Nov;114:530–536. doi: 10.3181/00379727-114-28724. [DOI] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES