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. 1969 Apr;17(4):497–503. doi: 10.1128/am.17.4.497-503.1969

Bioassay for Hamycin and Amphotericin B in Serum and Other Biological Fluids

Smith Shadomy 1, Judith A McCay 1, Stephen I Schwartz 1
PMCID: PMC377729  PMID: 5814426

Abstract

A bioassay suitable for measuring concentrations of the polyene antifungal agents hamycin and amphotericin B in biological fluids is described. By using Paecilomyces varioti as the indicator organism, sensitivity of the bioassay was found to be in the range of 0.01 to 0.02 μg/ml. A linear dose-response curve was obtained with amphotericin B; the curve for hamycin was curvilinear. In a series of assays, hamycin serum levels in the range of 0.01 to 3.5 μg/ml were measured; with amphotericin B, serum levels in the range of 0.015 to 0.175 μg/ml were measured in patients receiving orthodox intravenous medication and as high as 9.0 μg/ml in one patient treated with extraordinarily high doses of the drug.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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